Lymphocyte Activation Gene 3 (LAG3) Recombinant Protein | LAG3 recombinant protein

Recombinant Lymphocyte Activation Gene 3 (LAG3)

Cat. Num. AAA2009196

• Gene Names: LAG3; CD223
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Lymphocyte Activation Gene 3 (LAG3); Recombinant Lymphocyte Activation Gene 3 (LAG3); LAG3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TYRDGFN VSIMYNLTVL GLEPPTPLTV YAGAGSRVGL PCRLPAGVGT RSFLTAKWTP PGGGPDLLVT GDNGDFTLRL EDVSQAQAGT YTCHIHLQEQ QLNATVTLAI ITVTPKSFGS PGSLGKLLCE VTPVSGQERF VWSSLDTPSQ RSFSGPWLEA QEAQLLSQPW QCQLYQGERL LGAAVYFTEL SSPGAQRSGR APGALPAGHL LLFLILGVLS LLLLVTGAFG FHLWRRQWRP RRFSALE
• Sequence Length: 525

• Applicable Applications for LAG3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Thr244~Glu487 (Accession # P18627) with two N-terminal Tags, His-tag and T7-tag
• Predicted isoelectric point: 8.8
• Predicted Molecular Mass: 30.2kDa
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Residues: Thr244~Glu487 (Accession # P18627), with two N-terminal Tags, His-tag and T7-tag.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 167614500
• NCBI GeneID: 3902
• NCBI Accession #: NP_002277.4
• NCBI GenBank Nucleotide #: NM_002286.5
• UniProt Accession #: P18627; A8K7T9
• Molecular Weight: 34kDa
• NCBI Official Full Name: lymphocyte activation gene 3 protein
• NCBI Official Synonym Full Names: lymphocyte-activation gene 3
• NCBI Official Symbol: LAG3
• NCBI Official Synonym Symbols: CD223
• NCBI Protein Information: lymphocyte activation gene 3 protein
• UniProt Protein Name: Lymphocyte activation gene 3 protein
• Protein Family: Lymphocyte activation gene 3 protein
• UniProt Gene Name: LAG3
• UniProt Synonym Gene Names: FDC; LAG-3
• UniProt Entry Name: LAG3_HUMAN

NCBI Description

Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]

Uniprot Description

LAG3: Involved in lymphocyte activation. Binds to HLA class-II antigens.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.32

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: transmembrane receptor activity; antigen binding; MHC class II protein binding

Biological Process: cell surface receptor linked signal transduction; negative regulation of interleukin-2 biosynthetic process; positive regulation of natural killer cell mediated cytotoxicity; negative regulation of T cell activation