Human L1 cell adhesion molecule ELISA Kit | L1CAM elisa kit

Human Neural cell adhesion molecule ligand 1, NCAM-L1 ELISA Kit

Cat. Num. AAA700933

• Gene Names: L1CAM; S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
• Reactivity: Human
• Synonyms: L1 cell adhesion molecule; Human Neural cell adhesion molecule ligand 1; NCAM-L1 ELISA Kit; CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAML1; S10; SPG1; OTTHUMP00000025992; OTTHUMP00000063091; antigen identified by monoclonal antibody R1; neural cell adhesion molecule L1; L1CAM elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human NCAM-L1. No significant cross-reactivity or interference between human NCAM-L1 and analogues was observed.
• Sequence Length: 1257

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 1.25 ng/ml -80 ng/ml.
• Sensitivity: The minimum detectable dose of human NCAM-L1 is typically less than 0.31 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for L1CAM elisa kit

Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NCAM-L1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NCAM-L1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCAM-L1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCAM-L1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4557707
• NCBI GeneID: 3897
• NCBI Accession #: NP_000416.1
• NCBI GenBank Nucleotide #: NM_000425.4
• UniProt Accession #: P32004; Q8TA87; A0AV65; A4ZYW4; B2RMU7; G3XAF4
• Molecular Weight: 140,003 Da
• NCBI Official Full Name: neural cell adhesion molecule L1 isoform 1
• NCBI Official Synonym Full Names: L1 cell adhesion molecule
• NCBI Official Symbol: L1CAM
• NCBI Official Synonym Symbols: S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
• NCBI Protein Information: neural cell adhesion molecule L1; antigen identified by monoclonal antibody R1
• UniProt Protein Name: Neural cell adhesion molecule L1
• Protein Family: Neural cell adhesion molecule
• UniProt Gene Name: L1CAM
• UniProt Synonym Gene Names: CAML1; MIC5; N-CAM-L1; NCAM-L1
• UniProt Entry Name: L1CAM_HUMAN

NCBI Description

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]

Uniprot Description

Function: Cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons.