Guinea Pig L1-Cell Adhesion Molecule ELISA Kit | L1CAM elisa kit

Guinea pig L1-Cell Adhesion Molecule ELISA Kit

Cat. Num. AAA746752

• Gene Names: L1CAM; S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
• Reactivity: Guinea Pig
• Synonyms: L1-Cell Adhesion Molecule; Guinea pig L1-Cell Adhesion Molecule ELISA Kit; L1CAM elisa kit

• Reactivity: Guinea Pig

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This L1CAM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig L1CAM. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This L1CAM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig L1CAM. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for L1CAM elisa kit

Principle of the Assay: L1CAM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-L1CAM antibody and an L1CAM-HRP conjugate. The assay sample and buffer are incubated together with L1CAM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the L1CAM concentration since L1CAM from samples and L1CAM-HRP conjugate compete for the anti-L1CAM antibody binding site. Since the number of sites is limited, as more sites are occupied by L1CAM from the sample, fewer sites are left to bind L1CAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The L1CAM concentration in each sample is interpolated from this standard curve.

Product Categories/Family for L1CAM elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 19388023
• NCBI GeneID: 3897
• Molecular Weight: 138,908 Da
• NCBI Official Full Name: L1CAM protein, partial
• NCBI Official Synonym Full Names: L1 cell adhesion molecule
• NCBI Official Symbol: L1CAM
• NCBI Official Synonym Symbols: S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
• NCBI Protein Information: neural cell adhesion molecule L1; antigen identified by monoclonal antibody R1
• UniProt Protein Name: Neural cell adhesion molecule L1
• Protein Family: Neural cell adhesion molecule
• UniProt Gene Name: L1CAM
• UniProt Synonym Gene Names: CAML1; MIC5; N-CAM-L1; NCAM-L1
• UniProt Entry Name: L1CAM_HUMAN

NCBI Description

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]

Uniprot Description

NCAM-L1: cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons. Two splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: presynaptic membrane; focal adhesion; cell surface; integral to membrane; plasma membrane; terminal button; external side of plasma membrane

Molecular Function: integrin binding; identical protein binding; protein self-association; sialic acid binding

Biological Process: axon guidance; nervous system development; positive regulation of calcium-mediated signaling; chemotaxis; heterophilic cell adhesion; leukocyte adhesion; cell surface receptor linked signal transduction; homotypic cell-cell adhesion; cell-cell adhesion mediated by integrin; positive regulation of cell-cell adhesion; blood coagulation; cell adhesion; homophilic cell adhesion; leukocyte migration

Disease: Hydrocephalus Due To Congenital Stenosis Of Aqueduct Of Sylvius; Corpus Callosum, Partial Agenesis Of, X-linked; Masa Syndrome