Sheep Liver Fatty acid Binding Protein ELISA Kit | L-FABP elisa kit

Sheep Liver Fatty acid Binding Protein ELISA Kit

Cat. Num. AAA738607

• Gene Names: Fabp1; SCP; p14; SCP.; Fabplg; L-FABP
• Reactivity: Sheep
• Synonyms: Liver Fatty acid Binding Protein; Sheep Liver Fatty acid Binding Protein ELISA Kit; L-FABP elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for L-FABP elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This L-FABP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep L-FABP. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY L-FABP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-L-FABP antibody and an L-FABP-HRP conjugate. The assay sample and buffer are incubated together with L-FABP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the L-FABP concentration since L-FABP from samples and L-FABP-HRP conjugate compete for the anti-L-FABP antibody binding site. Since the number of sites is limited, as more sites are occupied by L-FABP from the sample, fewer sites are left to bind L-FABP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The L-FABP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for L-FABP elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 204086
• NCBI GeneID: 24360
• Molecular Weight: 14,273 Da
• NCBI Official Full Name: liver fatty acid binding protein
• NCBI Official Synonym Full Names: fatty acid binding protein 1, liver
• NCBI Official Symbol: Fabp1
• NCBI Official Synonym Symbols: SCP; p14; SCP.; Fabplg; L-FABP
• NCBI Protein Information: fatty acid-binding protein, liver; Z-protein; squalene- and sterol-carrier protein; liver-type fatty acid-binding protein
• UniProt Protein Name: Fatty acid-binding protein, liver
• UniProt Gene Name: Fabp1
• UniProt Synonym Gene Names: L-FABP; SCP
• UniProt Entry Name: FABPL_RAT

NCBI Description

binds oleic acid and other hydrophobic ligands; may play a role in hepatocyte cell proliferation; may transport activated chemical carcinogens [RGD, Feb 2006]

Uniprot Description

FABP1: Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding

Cellular Component: peroxisomal matrix; apical cortex; cytoplasm; cytosol; nucleus

Molecular Function: antioxidant activity; protein binding; bile acid binding; phospholipid binding; drug binding; long-chain fatty acid transporter activity; chromatin binding; lysophospholipid transporter activity; fatty acid binding

Biological Process: long-chain fatty acid transport; mitosis; transport; lysophospholipid transport; positive regulation of hydrolase activity; positive regulation of cell proliferation; negative regulation of caspase activity; intestinal absorption; positive regulation of fatty acid beta-oxidation; negative regulation of apoptosis