Human Kynurenine ELISA Kit | KYN elisa kit

Human Kynurenine, KYN ELISA Kit

Cat. Num. AAA704658

• Reactivity: Human
• Synonyms: Kynurenine; Human Kynurenine; KYN ELISA Kit; Human Kynurenine (KYN) ELISA Kit; OTTHUMP00000204304; l-kynurenine hydrolase; KYN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human KYN. No significant cross-reactivity or interference between human KYN and analogues was observed.
• Sequence Length: 307

• Samples: Serum, urine, tissue homogenates
• Assay Type: Quantitative Competitive
• Detection Range: 0.12pmol/ml-500pmol/ml
• Sensitivity: < 0.12pmol/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KYN elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are added to the appropriate microtiter plate wells with antibody specific for KYN and Horseradish Peroxidase (HRP) conjugated goat-anti-rabbit antibody. The competitive inhibition reaction is launched between with pre-coated KYN and KYN in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of KYN in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 74101437
• NCBI GeneID: 8942
• NCBI Accession #: NP_001028170.1
• NCBI GenBank Nucleotide #: NM_001032998.1
• UniProt Accession #: Q16719; Q6I9T2; Q9BVW3; B2RCZ5; D3DP79
• Molecular Weight: 52,352 Da
• NCBI Official Full Name: kynureninase isoform b
• NCBI Official Synonym Full Names: kynureninase
• NCBI Official Symbol: KYNU
• NCBI Protein Information: kynureninase; kynureninase (L-kynurenine hydrolase)
• UniProt Protein Name: Kynureninase
• Protein Family: Kynurenine formamidase
• UniProt Gene Name: KYNU
• UniProt Entry Name: KYNU_HUMAN

NCBI Description

Kynureninase is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Kynureninase is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]

Uniprot Description

Function: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity.UniRule annotationHAMAP-Rule:MF_03017

Catalytic activity: L-kynurenine + H2O = anthranilate + L-alanine.4 PublicationsManual assertion based on experiment in:Ref.1

Cofactor: Pyridoxal phosphate.

Enzyme regulation: Inhibited by o-methoxybenzoylalanine (OMBA).2 PublicationsManual assertion based on experiment in:Ref.1