Human keratin 35 ELISA Kit | KRT35 elisa kit
Human Keratin, type I cuticular Ha5, KRT35 ELISA Kit
Gene Names
KRT35; HA5; Ha-5; hHa5; KRTHA5
Reactivity
Human
Synonyms
keratin 35; Human Keratin; type I cuticular Ha5; KRT35 ELISA Kit; type I cuticular Ha5 (KRT35) ELISA kit; HA5; Ha-5; KRTHA5; hHa5; HHa5 hair keratin type I intermediate filament; hard keratin; type I; 5; keratin; hair; acidic; KRT35 elisa kit
Reactivity
Human
Specificity
No significant cross-reactivity or interference between Human KRT35 and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Assay Type
Sandwich
Detection Range
3.12 ng/ml - 100 ng/ml.
Sensitivity
1.0 ng/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated KRT35 concentrations in Human serum, plasma and other body fluids. Using Purified Human KRT35 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add KRT35 and KRT35 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of KRT35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Related Product Information for KRT35 elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated KRT35 concentrations in Human serum, plasma and other body fluids. Using Purified Human KRT35 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add KRT35 and KRT35 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of KRT35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,361 Da
NCBI Official Full Name
keratin, type I cuticular Ha5
NCBI Official Synonym Full Names
keratin 35
NCBI Official Symbol
KRT35
NCBI Official Synonym Symbols
HA5; Ha-5; hHa5; KRTHA5
NCBI Protein Information
keratin, type I cuticular Ha5; K35; keratin-35; hard keratin, type I, 5; hair keratin, type I Ha5; keratin, hair, acidic, 5; HHa5 hair keratin type I intermediate filament
UniProt Protein Name
Keratin, type I cuticular Ha5
Protein Family
UniProt Gene Name
KRT35
UniProt Synonym Gene Names
HHA5; HKA5; KRTHA5; K35
UniProt Entry Name
KRT35_HUMAN
NCBI Description
The protein encoded by this gene is a member of the keratin gene family. This type I hair keratin is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. [provided by RefSeq, Jul 2008]
Uniprot Description
KRTHA5: is a member of the keratin gene family. This type I hair keratin is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. [provided by RefSeq, Jul 2008]
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 17q21.2
Cellular Component: extracellular space; intermediate filament
Molecular Function: structural molecule activity
Biological Process: anatomical structure morphogenesis
Research Articles on KRT35
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Product Notes
The Human KRT35 krt35 (Catalog #AAA9337769) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9337769 ELISA Kit recognizes Human KRT35. It is sometimes possible for the material contained within the vial of "keratin 35, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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