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Testing Data (P-peptide-+)

anti-Human Keratin 18 Antibody | anti-KRT18 antibody

Keratin 18 (Phospho-Ser33) Antibody

Gene Names
KRT18; K18; CK-18; CYK18
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
Keratin 18; Keratin 18 (Phospho-Ser33) Antibody; CK 18; CK18; CYK18; Cytokeratin endo B; K18; K1C18; KERD; KRT1-18; Keratin; Keratin D; type I cytoskeletal 18; cytokeratin 18; anti-KRT18 antibody
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Keratin 18 (phospho-Ser33) antibody detects endogenous levels of Keratin 18 only when phosphorylated at serine 33.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
430
Applicable Applications for anti-KRT18 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Keratin 18 around the phosphorylation site of serine 33 (A-A-SP-V-Y).
All Sites
Human: Ser33
Preparation and Storage
Store at-20 degree C for 1 year

Testing Data

(P-peptide-+)

Testing Data (P-peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 18 (phospho-Ser33) antibody. Western blot analysis of extracts from HT29 cells, using Keratin 18 (phospho-Ser33) antibody.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 18 (phospho-Ser33) antibody. Western blot analysis of extracts from HT29 cells, using Keratin 18 (phospho-Ser33) antibody.)

Immunofluorescence (IF)

(P-peptide-+ Immunofluorescence analysis of HeLa cells, using Keratin 18 (phospho-Ser33) antibody (#11316).)

Immunofluorescence (IF) (P-peptide-+ Immunofluorescence analysis of HeLa cells, using Keratin 18 (phospho-Ser33) antibody (#11316).)
References
J Liao, J. Cell Biol., Dec 1995; 131: 1291. CF Chou and MB Omary, J. Biol. Chem., Feb 1993; 268: 4465-4472. NO Ku and MB Omary, J. Cell Biol., Oct 1994; 127: 161. Nam-On Ku J. Cell Biol., May 2000; 149: 547.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48,058 Da
NCBI Official Full Name
keratin, type I cytoskeletal 18
NCBI Official Synonym Full Names
keratin 18
NCBI Official Symbol
KRT18
NCBI Official Synonym Symbols
K18; CK-18; CYK18
NCBI Protein Information
keratin, type I cytoskeletal 18
UniProt Protein Name
Keratin, type I cytoskeletal 18
Protein Family
UniProt Gene Name
KRT18
UniProt Synonym Gene Names
CYK18; CK-18; K18

NCBI Description

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Research Articles on KRT18

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Product Notes

The KRT18 krt18 (Catalog #AAA8509312) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Keratin 18 (Phospho-Ser33) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin 18 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000. Researchers should empirically determine the suitability of the KRT18 krt18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Keratin 18, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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