Human Keratin, type I cytoskeletal 16 ELISA Kit | KRT16 elisa kit

Human Keratin, type I cytoskeletal 16 ELISA Kit

Cat. Num. AAA761367

• Gene Names: KRT16; K16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A
• Reactivity: Human
• Synonyms: Keratin; type I cytoskeletal 16; Human Keratin; type I cytoskeletal 16 ELISA Kit; KRT16/Cytokeratin-16 (CK-16)/Keratin-16/CK16/K1CP/NEPPK/FNEPPK/KRT16A; KRT16 elisa kit

• Reactivity: Human
• Sequence Length: 473

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for KRT16 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human KRT16 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human KRT16 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human KRT16 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman KRT16 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 24430192
• NCBI GeneID: 3868
• NCBI Accession #: NP_005548.2
• NCBI GenBank Nucleotide #: NM_005557.3
• Molecular Weight: 51,268 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 16
• NCBI Official Synonym Full Names: keratin 16, type I
• NCBI Official Symbol: KRT16
• NCBI Official Synonym Symbols: K16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A
• NCBI Protein Information: keratin, type I cytoskeletal 16
• UniProt Protein Name: Keratin, type I cytoskeletal 16
• Protein Family: Keratin
• UniProt Gene Name: KRT16
• UniProt Synonym Gene Names: KRT16A; CK-16; K16
• UniProt Entry Name: K1C16_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq, Jul 2008]

Uniprot Description

K16: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K6. K16 and K17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoskeleton; intermediate filament; nucleus

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: aging; cell proliferation; cytoskeleton organization and biogenesis; epidermis development; hair cycle; inflammatory response; innate immune response; intermediate filament cytoskeleton organization and biogenesis; keratinization; keratinocyte differentiation; keratinocyte migration; morphogenesis of an epithelium; negative regulation of cell migration

Disease: Pachyonychia Congenita 1; Palmoplantar Keratoderma, Nonepidermolytic, Focal