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SDS-Page (SDS-PAGE)

Cytokeratin 13 (CK13) Recombinant Protein | CK13 recombinant protein

Recombinant Cytokeratin 13 (CK13)

Gene Names
KRT13; K13; CK13; WSN2
Applications
SDS-Page, Western Blot
Purity
Greater than 90% by SDS-PAGE
Synonyms
Cytokeratin 13 (CK13); Recombinant Cytokeratin 13 (CK13); CK13/Cytokeratin 13; KRT13; Keratin 13; CK13 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
Greater than 90% by SDS-PAGE
Form/Format
Freeze-dried powder
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Sequence
Glu104~Ala403
Applicable Applications for CK13 recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Tag
Two N-terminal Tags, His-tag and SUMO-tag
Expression System
Prokaryotic expression
Organism Species
Human
Isoelectric Point
4.3
Subcellular Location
Nucleus
Reconstitution
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

SDS-Page

(SDS-PAGE)

SDS-Page (SDS-PAGE)
Product Categories/Family for CK13 recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
38,581 Da
NCBI Official Full Name
cytokeratin 13
NCBI Official Synonym Full Names
keratin 13
NCBI Official Symbol
KRT13
NCBI Official Synonym Symbols
K13; CK13; WSN2
NCBI Protein Information
keratin, type I cytoskeletal 13
UniProt Protein Name
Keratin, type I cytoskeletal 13
UniProt Gene Name
KRT13
UniProt Synonym Gene Names
CK-13; K13

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

MiscellaneousThere are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Research Articles on CK13

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Product Notes

The CK13 krt13 (Catalog #AAA2125511) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cytokeratin 13 (CK13) can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, Western Blot (WB). Researchers should empirically determine the suitability of the CK13 krt13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Glu104~Ala 403. It is sometimes possible for the material contained within the vial of "Cytokeratin 13 (CK13), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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