Kallikrein 11 (KLK11) Recombinant Protein | KLK11 recombinant protein

Recombinant Kallikrein 11 (KLK11)

Cat. Num. AAA2018657

• Gene Names: KLK11; TLSP; PRSS20
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Kallikrein 11 (KLK11); Recombinant Kallikrein 11 (KLK11); KLK11 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- IL LALATGLVGG ETRIIKGFEC KPHSQPWQAA LFEKTRLLCG ATLIAPRWLL TAAHCLKPRY IVHLGQHNLQ KEEGCEQTRT ATESFPHPGF NNSLPNKDHR NDIMLVKMAS PVSITWAVRP LTLSSRCVTA GTSCLISGWG STSSPQLRLP HTLRCANITI IEHQKCENAY PGNITDTMVC ASVQEGGKDS CQGDSGGPLVCNQSLQGIIS WGQDPCAITR KPGVYTKVCK YVDWIQETMK NN
• Sequence Length: 250

• Applicable Applications for KLK11 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ile39~Asn282 (Accession # Q9UBX7) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Secreted. Golgi apparatus
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8.8
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 209862865
• NCBI GeneID: 11012
• NCBI Accession #: NP_001129504.1
• NCBI GenBank Nucleotide #: NM_001136032.2
• UniProt Accession #: Q9UBX7; O75837; Q0WXX5; Q8IXD7; Q9NS65
• Molecular Weight: 30.5kDa
• NCBI Official Full Name: kallikrein-11 isoform 1
• NCBI Official Synonym Full Names: kallikrein-related peptidase 11
• NCBI Official Symbol: KLK11
• NCBI Official Synonym Symbols: TLSP; PRSS20
• NCBI Protein Information: kallikrein-11; hK11; hippostasin; kallikrein 11; serine protease 20; trypsin-like protease
• UniProt Protein Name: Kallikrein-11
• Protein Family: Kallikrein
• UniProt Gene Name: KLK11
• UniProt Synonym Gene Names: PRSS20; TLSP; hK11
• UniProt Entry Name: KLK11_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in multiple transcript variants encoding distinct isoforms which are differentially expressed.[provided by RefSeq, Nov 2009]

Uniprot Description

KLK11: Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. Belongs to the peptidase S1 family. Kallikrein subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Protease; EC 3.4.21.-; Secreted

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: Golgi apparatus; extracellular space

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: proteolysis