Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Alcohol dehydrogenase 6 (ADH6) Standard Curve.)

Human Alcohol Dehydrogenase 6 (ADH6) ELISA Kit | ADH6 elisa kit

Human Alcohol Dehydrogenase 6 (ADH6) ELISA Kit

Gene Names
ADH6; ADH-5
Reactivity
Human
Synonyms
Alcohol Dehydrogenase 6 (ADH6); Human Alcohol Dehydrogenase 6 (ADH6) ELISA Kit; ADH6; ADH-5; aldehyde reductase; class V alcohol dehydrogenase; ADH6 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human Alcohol dehydrogenase 6 (ADH6) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADH6. No significant cross-reactivity or interference between Human ADH6 and analogues was observed.
Sequence Length
368
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Alcohol dehydrogenase 6 (ADH6) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Alcohol dehydrogenase 6 (ADH6) Standard Curve.)
Related Product Information for ADH6 elisa kit
Description: This Human Alcohol dehydrogenase 6 (ADH6) ELISA Kit employs a two-site sandwich ELISA to quantitate ADH6 in samples. An antibody specific for ADH6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADH6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADH6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADH6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Alcohol dehydrogenase 6 is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This gene is expressed in the stomach as well as in the liver, and it contains a glucocorticoid response element upstream of its 5' UTR, which is a steroid hormone receptor binding site. The deduced amino acid sequence of the open reading frame of this gene shows about 60% positional identity with other known alcohol dehydrogenases. This gene may have a distinct physiologic function.The ADH6 sequence shows approximatley 60% positional identity with known human ADHs, suggesting interclass similarity within the human ADH family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
130
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
alcohol dehydrogenase 6 isoform 2
NCBI Official Synonym Full Names
alcohol dehydrogenase 6 (class V)
NCBI Official Symbol
ADH6
NCBI Official Synonym Symbols
ADH-5
NCBI Protein Information
alcohol dehydrogenase 6
UniProt Protein Name
Alcohol dehydrogenase 6
Protein Family
UniProt Gene Name
ADH6
UniProt Entry Name
ADH6_HUMAN

NCBI Description

This gene encodes class V alcohol dehydrogenase, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This gene is expressed in the stomach as well as in the liver, and it contains a glucocorticoid response element upstream of its 5' UTR, which is a steroid hormone receptor binding site. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ADH6: class V alcohol dehydrogenase, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This gene is expressed in the stomach as well as in the liver, and it contains a glucocorticoid response element upstream of its 5' UTR, which is a steroid hormone receptor binding site. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cofactor and Vitamin Metabolism - retinol; Amino Acid Metabolism - tyrosine; Oxidoreductase; Lipid Metabolism - fatty acid; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.1.1.1

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: cytosol

Molecular Function: zinc ion binding; alcohol dehydrogenase activity; ethanol binding; alcohol dehydrogenase activity, zinc-dependent

Biological Process: response to ethanol; xenobiotic metabolic process; ethanol oxidation

Research Articles on ADH6

Similar Products

Product Notes

The Human ADH6 adh6 (Catalog #AAA9714602) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714602 ELISA Kit recognizes Human ADH6. It is sometimes possible for the material contained within the vial of "Alcohol Dehydrogenase 6 (ADH6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.