Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat CXXC4 Polyclonal Antibody | anti-CXXC4 antibody
Goat anti-CXXC4 Antibody
Immunohistochemistry: In paraffin embedded Human Breast shows cystoplasm staining in myoepithelial cells. Recommended concentration, 3-5ug/ml.
Western Blot: Preliminary experiments in Human Brain (Cerebellum, Hippocampus) lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a CXXC-type zinc finger domain-containing protein that functions as an antagonist of the canonical wingless/integrated signaling pathway. The encoded protein negatively regulates wingless/integrated signaling through interaction with the post synaptic density protein/ Drosophila disc large tumor suppressor/ zonula occludens-1 protein domain of Dishevelled, a scaffolding protein required for the stabilization of the transcriptional co-activator beta-catenin. In addition, the CXXC domain of this protein has been shown to bind unmethylated CpG dinucleotides, localize to promoters and CpG islands, and interact with the catalytic domain of methylcytosine dioxygenase ten-eleven-translocation 2, an iron and alpha-ketoglutarate-dependent dioxygenase that modifies the methylation status of DNA. In humans, a mutation in this gene has been associated with development of malignant renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Uniprot Description
CXXC4: Acts as a negative regulator of the Wnt signaling pathway via its interaction with DVL1.
Protein type: Inhibitor
Chromosomal Location of Human Ortholog: 4q24
Cellular Component: cytoplasm; cytoplasmic vesicle
Molecular Function: DNA binding; PDZ domain binding; zinc ion binding
Biological Process: negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; zygotic determination of dorsal/ventral axis
Research Articles on CXXC4
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Product Notes
The CXXC4 cxxc4 (Catalog #AAA421859) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CXXC4 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CXXC4 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Breast shows cystoplasm staining in myoepithelial cells. Recommended concentration, 3-5ug/ml. Western Blot: Preliminary experiments in Human Brain (Cerebellum, Hippocampus) lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). Researchers should empirically determine the suitability of the CXXC4 cxxc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GTSLERTPVP SAEA. It is sometimes possible for the material contained within the vial of "CXXC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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