Human Potassium channel subfamily K member 18 ELISA Kit | KCNK18 elisa kit
Human Potassium channel subfamily K member 18 (KCNK18) ELISA Kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate KCNK18 in Human serum, plasma, tissue homogenates. An antibody specific for KCNK18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KCNK18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KCNK18 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KCNK18 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
Potassium channels play a role in many cellular processes including maintenance of the action potential, muscle contraction, hormone secretion, osmotic regulation, and ion flow. This gene encodes a member of the superfamily of potassium channel proteins containing two pore-forming P domains and the encoded protein functions as an outward rectifying potassium channel. A mutation in this gene has been found to be associated with migraine with aura.[provided by RefSeq, Jan 2011]
Uniprot Description
KCNK18: Outward rectifying potassium channel. Produces rapidly activating outward rectifier K(+) currents. May function as background potassium channel that sets the resting membrane potential. Channel activity is directly activated by calcium signal. Activated by the G(q)-protein coupled receptor pathway. The calcium signal robustly activates the channel via calcineurin, whereas the anchoring of 14-3-3/YWHAH interferes with the return of the current to the resting state after activation. Inhibited also by arachidonic acid and other naturally occurring unsaturated free fatty acids. Channel activity is also enhanced by volatile anesthetics, such as isoflurane. Appears to be the primary target of hydroxy-alpha-sanshool, an ingredient of Schezuan pepper. May be involved in the somatosensory function with special respect to pain sensation. Interacts with calcineurin. Interacts with YWHAH, in a phosphorylation-dependent manner. Expressed specifically in dorsal root ganglion and trigeminal ganglion neurons. Detected at low levels in spinal cord. Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.
Protein type: Channel, ligand-gated; Channel, potassium; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 10q25.3
Cellular Component: integral component of plasma membrane; plasma membrane
Molecular Function: calcium-activated potassium channel activity; outward rectifier potassium channel activity; potassium channel activity; potassium ion leak channel activity
Biological Process: potassium ion transport; stabilization of membrane potential
Disease: Migraine, With Or Without Aura, Susceptibility To, 13
Research Articles on KCNK18
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Product Notes
The Human KCNK18 kcnk18 (Catalog #AAA280534) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280534 ELISA Kit recognizes Human KCNK18. It is sometimes possible for the material contained within the vial of "Potassium channel subfamily K member 18, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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