Human ATP-sensitive inward rectifier potassium channel 10 ELISA Kit | KCNJ10 elisa kit

Human ATP-sensitive inward rectifier potassium channel 10 ELISA Kit

Cat. Num. AAA764610

• Gene Names: KCNJ10; KIR1.2; KIR4.1; SESAME; BIRK-10; KCNJ13-PEN
• Reactivity: Human
• Synonyms: ATP-sensitive inward rectifier potassium channel 10; Human ATP-sensitive inward rectifier potassium channel 10 ELISA Kit; KCNJ10/ATP-dependent inwardly rectifying potassium channel Kir4.1/Inward rectifier K (+) channel Kir1.2/Potassium channel/inwardly; KCNJ10 elisa kit

• Reactivity: Human
• Sequence Length: 379

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for KCNJ10 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human KCNJ10 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human KCNJ10 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human KCNJ10 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human KCNJ10 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 25121966
• NCBI GeneID: 3766
• NCBI Accession #: NP_002232.2
• NCBI GenBank Nucleotide #: NM_002241.4
• Molecular Weight: 42,508 Da
• NCBI Official Full Name: ATP-sensitive inward rectifier potassium channel 10
• NCBI Official Synonym Full Names: potassium voltage-gated channel subfamily J member 10
• NCBI Official Symbol: KCNJ10
• NCBI Official Synonym Symbols: KIR1.2; KIR4.1; SESAME; BIRK-10; KCNJ13-PEN
• NCBI Protein Information: ATP-sensitive inward rectifier potassium channel 10
• UniProt Protein Name: ATP-sensitive inward rectifier potassium channel 10
• Protein Family: ATP-sensitive inward rectifier potassium channel
• UniProt Gene Name: KCNJ10
• UniProt Entry Name: KCJ10_HUMAN

NCBI Description

This gene encodes a member of the inward rectifier-type potassium channel family, characterized by having a greater tendency to allow potassium to flow into, rather than out of, a cell. The encoded protein may form a heterodimer with another potassium channel protein and may be responsible for the potassium buffering action of glial cells in the brain. Mutations in this gene have been associated with seizure susceptibility of common idiopathic generalized epilepsy syndromes. [provided by RefSeq, Jul 2008]

Uniprot Description

Kir4.1: May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Defects in KCNJ10 are the cause of seizures, sensorineural deafness, ataxia, mental retardation, and electrolyte imbalance (SESAMES). A complex disorder characterized by generalized seizures with onset in infancy, delayed psychomotor development, ataxia, sensorineural hearing loss, hypokalemia, metabolic alkalosis, and hypomagnesemia. Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ10 subfamily.

Protein type: Channel, potassium; Membrane protein, integral; Membrane protein, multi-pass; Channel, ligand-gated

Chromosomal Location of Human Ortholog: 1q23.2

Cellular Component: basolateral plasma membrane; integral to plasma membrane; plasma membrane

Molecular Function: ATP binding; ATP-activated inward rectifier potassium channel activity; protein binding

Biological Process: adult walking behavior; glutamate uptake during transmission of nerve impulse; myelination in the central nervous system; potassium ion homeostasis; potassium ion import; potassium ion transport; regulation of long-term neuronal synaptic plasticity; regulation of resting membrane potential; synaptic transmission; visual perception

Disease: Deafness, Autosomal Recessive 4, With Enlarged Vestibular Aqueduct; Pendred Syndrome; Seizures, Sensorineural Deafness, Ataxia, Mental Retardation, And Electrolyte Imbalance