Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 Recombinant Protein | KAZALD1 recombinant protein

Recombinant Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1)

Cat. Num. AAA2031159

• Gene Names: KAZALD1; BONO1; FKSG28; FKSG40; IGFBP-rP10
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Kazal Type Serine Protease Inhibitor Domain Containing Protein 1; Recombinant Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1); KAZALD1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; RPSPGPDYLR RGWMRLLAEGEGCAPCRPEE CAAPRGCLAG RVRDACGCCW ECANLEGQLC DLDPSAHFYGHCGEQLECRL DTGGDLSRGE VPEPLCACRS QSPLCGSDGH TYSQICRLQEAARARPDANL TVAHPGPCES GPQIVSHPYD TWNVTGQDVI FGCEVFAYPMASIEWRKDGL DIQLPGDDPH ISVQFRGGPQ RFEVTGWLQI QAVRPSDEGTYRCLGRNALG QVEAPASLTV LTPDQLNSTG IPQLRSLNLV PEEEAESEENDDYY
• Sequence Length: 304

• Applicable Applications for KAZALD1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 33.7kDa
• Accurate Molecular Mass (KD): 37kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Arg31~Tyr304
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 19923632
• NCBI GeneID: 81621
• NCBI Accession #: NP_112191.2
• NCBI GenBank Nucleotide #: NM_030929.4
• Molecular Weight: 26,525 Da
• NCBI Official Full Name: kazal-type serine protease inhibitor domain-containing protein 1
• NCBI Official Synonym Full Names: Kazal-type serine peptidase inhibitor domain 1
• NCBI Official Symbol: KAZALD1
• NCBI Official Synonym Symbols: BONO1; FKSG28; FKSG40; IGFBP-rP10
• NCBI Protein Information: kazal-type serine protease inhibitor domain-containing protein 1
• UniProt Protein Name: Kazal-type serine protease inhibitor domain-containing protein 1
• Protein Family: Kazal-type serine protease inhibitor domain-containing protein
• UniProt Gene Name: KAZALD1
• UniProt Entry Name: KAZD1_HUMAN

NCBI Description

This gene encodes a secreted member of the insulin growth factor-binding protein (IGFBP) superfamily. It contains an N-terminal insulin growth factor-binding domain, a central Kazal-type serine protease inhibitor and follistatin-like domain, and a C-terminal immunoglobulin-like domain. Studies of the mouse ortholog suggest that this gene product may have a function in bone development and bone regeneration. [provided by RefSeq, Feb 2009]

Uniprot Description

KAZALD1: Involved in the proliferation of osteoblasts during bone formation and bone regeneration. Promotes matrix assembly. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q24.31

Molecular Function: insulin-like growth factor binding

Biological Process: extracellular matrix organization and biogenesis; ossification; multicellular organismal development; regulation of cell growth; cell differentiation