Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein S (PROS) Standard Curve.)
Human Protein S (PROS) ELISA Kit | PROS elisa kit
Human Protein S (PROS) ELISA Kit
Gene Names
PROS1; PSA; PROS; PS21; PS22; PS23; PS24; PS25; THPH5; THPH6
Reactivity
Human
Synonyms
Protein S (PROS); Human Protein S (PROS) ELISA Kit; PROS; PSA; PS; PS26; PS21; PS22; PS23; PS24; PS25; VKDPS; VKDP-S; Protein Sa; Vitamin K Dependent Protein S; PROS elisa kit
Reactivity
Human
Specificity
Human Protein S (PROS) ELISA Kit has high sensitivity and excellent specificity for detection of Human PROS. No significant cross-reactivity or interference between Human PROS and analogues was observed.
Sequence Length
650
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein S (PROS) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein S (PROS) Standard Curve.)
Related Product Information for PROS elisa kit
Description: This Human Protein S (PROS) ELISA Kit employs a two-site sandwich ELISA to quantitate PROS in samples. An antibody specific for PROS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PROS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Protein S is a vitamin K-dependent plasma glycoprotein synthesized in the endothelium. In the circulation, Protein S exists in two forms: a free form and a complex form bound to complement protein C4b. The best characterized function of Protein S is its role in the anti coagulation pathway, it functions as a cofactor to Protein C in the inactivation of Factors Va and VIIIa. Only the free form has cofactor activity. Protein S can bind to negatively charged phospholipids via the carboxylated GLA domain. This property allows Protein S to function in the removal of cells which are undergoing apoptosis. Apoptosis is a form of cell death that is used by the body to remove unwanted or damaged cells from tissues.
Background: Protein S is a vitamin K-dependent plasma glycoprotein synthesized in the endothelium. In the circulation, Protein S exists in two forms: a free form and a complex form bound to complement protein C4b. The best characterized function of Protein S is its role in the anti coagulation pathway, it functions as a cofactor to Protein C in the inactivation of Factors Va and VIIIa. Only the free form has cofactor activity. Protein S can bind to negatively charged phospholipids via the carboxylated GLA domain. This property allows Protein S to function in the removal of cells which are undergoing apoptosis. Apoptosis is a form of cell death that is used by the body to remove unwanted or damaged cells from tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
protein S, partial
NCBI Official Synonym Full Names
protein S
NCBI Official Symbol
PROS1
NCBI Official Synonym Symbols
PSA; PROS; PS21; PS22; PS23; PS24; PS25; THPH5; THPH6
NCBI Protein Information
vitamin K-dependent protein S
Protein Family
NCBI Description
This gene encodes a vitamin K-dependent plasma protein that functions as a cofactor for the anticoagulant protease, activated protein C (APC) to inhibit blood coagulation. It is found in plasma in both a free, functionally active form and also in an inactive form complexed with C4b-binding protein. Mutations in this gene result in autosomal dominant hereditary thrombophilia. An inactive pseudogene of this locus is located at an adjacent region on chromosome 3. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Oct 2015]
Research Articles on PROS
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Product Notes
The Human PROS (Catalog #AAA9714786) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714786 ELISA Kit recognizes Human PROS. It is sometimes possible for the material contained within the vial of "Protein S (PROS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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