Testing Data
(Immunofluorescence staining of methanol-fixed HeLa cells using Keratin 8 (Phospho-Ser74) Antibody (E011307, Red).)
anti-Human Keratin 8 Antibody | anti-K8 antibody
Keratin 8 (Phospho-Ser74) Antibody
Gene Names
KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
Reactivity
Human
Applications
Immunofluorescence
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
Keratin 8; Keratin 8 (Phospho-Ser74) Antibody; anti-K8 antibody
Reactivity
Human
Specificity
Keratin 8 (Phospho-Ser74) Antibody detects endogenous levels of Keratin 8 only whenphosphorylated at serine 74.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
483
Applicable Applications for anti-K8 antibody
Immunofluorescence (IF)
Application Notes
IF: 1:100-1:200
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Keratin 8 around the phosphorylation site of serine 74 (L-L-SP-P-L).
Preparation and Storage
Store at-20 degree C for 1 year
Testing Data
(Immunofluorescence staining of methanol-fixed HeLa cells using Keratin 8 (Phospho-Ser74) Antibody (E011307, Red).)
Testing Data
(Immunofluorescence staining of methanol-fixed HeLa cells using Keratin 8 (Phospho-Ser74) Antibody (E011307, Red).)
Product Categories/Family for anti-K8 antibody
References
Nakamichi I, et al. (2002) Mol Biol Cell; 13(10): 3441-3451. Prochasson P, et al. (2002) Nucleic Acids Res; 30(15): 3312-3322. Pankov R, et al. (1994) Proc Natl Acad Sci U S A; 91(3): 873-877. Thorey IS, et al. (1993) Mol Cell Biol; 13(11): 6742-6751.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56,608 Da
NCBI Official Full Name
keratin, type II cytoskeletal 8 isoform 2
NCBI Official Synonym Full Names
keratin 8
NCBI Official Symbol
KRT8
NCBI Official Synonym Symbols
K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
NCBI Protein Information
keratin, type II cytoskeletal 8
UniProt Protein Name
Keratin, type II cytoskeletal 8
Protein Family
UniProt Gene Name
KRT8
UniProt Synonym Gene Names
CYK8; CK-8; K8
NCBI Description
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
Uniprot Description
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Research Articles on K8
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Product Notes
The K8 krt8 (Catalog #AAA856901) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Keratin 8 (Phospho-Ser74) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin 8 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF). IF: 1:100-1:200. Researchers should empirically determine the suitability of the K8 krt8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Keratin 8, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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