Janus Kinase 2 (JAK2) Recombinant Protein | JAK2 recombinant protein

Recombinant Janus Kinase 2 (JAK2)

Cat. Num. AAA2012040

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Janus Kinase 2 (JAK2); Recombinant Janus Kinase 2 (JAK2); JAK2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-TG NPPFIKLSDP GISITVLPKD ILQERIPWVP PECIENPKNL TLATDKWSFG TTLWEICSGG DKPLSALDSQ RKLQFYEDKH QLPAPKWTEL ANLINTCMDY EPDFRPAFRA VIRDLNS
• Sequence Length: 1132

• Applicable Applications for JAK2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Thr689~Ser807 (Accession # Q62689) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 13928696
• NCBI GeneID: 24514
• NCBI Accession #: NP_113702.1
• NCBI GenBank Nucleotide #: NM_031514.1
• UniProt Accession #: Q62689
• Molecular Weight: 15.0kDa
• NCBI Official Full Name: tyrosine-protein kinase JAK2
• NCBI Official Synonym Full Names: Janus kinase 2
• NCBI Official Symbol: Jak2
• NCBI Protein Information: tyrosine-protein kinase JAK2; JAK-2; Janus kinase 2 (a protein tyrosine kinase)
• UniProt Protein Name: Tyrosine-protein kinase JAK2
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Jak2
• UniProt Synonym Gene Names: JAK-2
• UniProt Entry Name: JAK2_RAT

NCBI Description

associated with receptors for erythropoietin, prolactin, growth hormones and interleukin-3; phosphorylated when activated by cytokines [RGD, Feb 2006]

Uniprot Description

JAK2: a non-receptor tyrosine-kinase involved in a specific subset of cytokine receptor signaling pathways, including IL-3, -5 and GM-CSF. Interacts with IL23R, SKB1 and STAM2. It has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. Fusion of Jak2 to TEL1 (ETV6) by t(9;12)(p24;p13) causes myeloproliferative disease in humans and mouse models. The Jak inhibitor AG490 inhibits constitutive Jak2 phosphorylation and causes apoptosis in cells from breast cancer and relapsing acute lymphoblastic leukemia. A single activating mutation is associated with several hematological malignancies. Inhibitor: AG490.

Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; EC 2.7.10.2; Kinase, protein; Oncoprotein; TK group; JakA family

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; nuclear matrix; cytoplasm; endomembrane system; intracellular; caveola; cytosol; nucleus; lipid raft

Molecular Function: protein C-terminus binding; histone binding; acetylcholine receptor binding; non-membrane spanning protein tyrosine kinase activity; protein kinase binding; interleukin-12 receptor binding; protein binding; growth hormone receptor binding; peptide hormone receptor binding; insulin receptor substrate binding; protein-tyrosine kinase activity; heme binding; phosphoinositide 3-kinase binding; SH2 domain binding; type 1 angiotensin receptor binding; ATP binding; receptor binding

Biological Process: positive regulation of nitric oxide biosynthetic process; activation of MAPKK activity; positive regulation of apoptosis; tyrosine phosphorylation of Stat3 protein; response to lipopolysaccharide; tyrosine phosphorylation of JAK2 protein; enzyme linked receptor protein signaling pathway; positive regulation of tyrosine phosphorylation of Stat3 protein; response to antibiotic; elevation of cytosolic calcium ion concentration; tumor necrosis factor-mediated signaling pathway; erythrocyte differentiation; negative regulation of neuron apoptosis; positive regulation of DNA binding; positive regulation of cell activation; positive regulation of protein import into nucleus, translocation; axon regeneration; positive regulation of insulin secretion; positive regulation of tumor necrosis factor production; JAK-STAT cascade; tyrosine phosphorylation of Stat5 protein; positive regulation of phosphoinositide 3-kinase cascade; tyrosine phosphorylation of STAT protein; positive regulation of peptidyl-tyrosine phosphorylation; response to hydroperoxide; mineralocorticoid receptor signaling pathway; tyrosine phosphorylation of Stat1 protein; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; response to oxidative stress; positive regulation of cell differentiation; positive regulation of nitric-oxide synthase biosynthetic process; negative regulation of apoptosis; positive regulation of phosphoprotein phosphatase activity; peptidyl-tyrosine phosphorylation; hormone-mediated signaling; negative regulation of heart contraction; protein amino acid autophosphorylation; platelet-derived growth factor receptor signaling pathway; signal transduction; host programmed cell death induced by symbiont; negative regulation of cell proliferation; positive regulation of cell proliferation; cell differentiation; STAT protein nuclear translocation; caspase activation; cytokine and chemokine mediated signaling pathway; negative regulation of DNA binding; positive regulation of interleukin-1 beta production; G-protein coupled receptor protein signaling pathway; positive regulation of tyrosine phosphorylation of Stat5 protein; regulation of inflammatory response; myeloid cell differentiation; negative regulation of cell-cell adhesion; induction of apoptosis by oxidative stress; positive regulation of cell migration; positive regulation of inflammatory response