Human integrin, beta 7 ELISA Kit | ITGB7 elisa kit

Human Integrin beta-7, ITGB7 ELISA Kit

Cat. Num. AAA919623

• Reactivity: Human
• Synonyms: integrin; beta 7; Human Integrin beta-7; ITGB7 ELISA Kit; Human Integrin beta-7 (ITGB7) ELISA kit; integrin beta 7 subunit; ITGB7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human ITGB7. No significant cross-reactivity or interference between human ITGB7 and analogues was observed.
• Sequence Length: 798

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312 ng/ml -20 ng/ml
• Sensitivity: < 0.078 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Related Product Information for ITGB7 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ITGB7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ITGB7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ITGB7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ITGB7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4504777
• NCBI GeneID: 3695
• NCBI Accession #: NP_000880.1
• NCBI GenBank Nucleotide #: NM_000889.2
• UniProt Accession #: P26010; Q9UCP7; Q9UCS7
• Molecular Weight: 86,903 Da
• NCBI Official Full Name: integrin beta-7
• NCBI Official Synonym Full Names: integrin, beta 7
• NCBI Official Symbol: ITGB7
• NCBI Protein Information: integrin beta-7; integrin beta 7 subunit; gut homing receptor beta subunit
• UniProt Protein Name: Integrin beta-7
• Protein Family: Integrin
• UniProt Gene Name: ITGB7
• UniProt Entry Name: ITB7_HUMAN

NCBI Description

This gene encodes a protein that is a member of the integrin superfamily. Members of this family are adhesion receptors that function in signaling from the extracellular matrix to the cell. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The encoded protein forms dimers with an alpha4 chain or an alphaE chain and plays a role in leukocyte adhesion. Dimerization with alpha4 forms a homing receptor for migration of lymphocytes to the intestinal mucosa and Peyer's patches. Dimerization with alphaE permits binding to the ligand epithelial cadherin, a calcium-dependent adhesion molecule. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]

Uniprot Description

ITGB7: integrin alpha-4/beta-7 (Peyer's patches-specific homing receptor lpam-1) is expected to play a role in adhesive interactions of leukocytes. It is a receptor for fibronectin and recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Integrin alpha-4/beta-7 is also a receptor for MADCAM1 and VCAM1. It recognizes the sequence L-D-T in MADCAM 1. Integrin alpha-e/beta-7 (HML-1) is a receptor for e-cadherin. Two alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Receptor, misc.; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cell surface; membrane; plasma membrane; integrin complex; receptor complex

Molecular Function: viral receptor activity; protein binding; metal ion binding; cell adhesion molecule binding

Biological Process: integrin-mediated signaling pathway; heterotypic cell-cell adhesion; regulation of immune response; entry of virus into host cell; extracellular matrix organization and biogenesis; multicellular organismal development; cell-matrix adhesion; cell adhesion; leukocyte tethering or rolling; receptor clustering