pro-ISG15 recombinant protein

pro-ISG15 (human), (recombinant) (His-tag)

Cat. Num. AAA566730

• Gene Names: ISG15; G1P2; IP17; UCRP; IFI15; hUCRP
• Purity: >=95% (SDS-PAGE)
• Synonyms: pro-ISG15; pro-ISG15 (human); (recombinant) (His-tag); pro-ISG15 recombinant protein

• Host: E Coli
• Purity/Purification: >=95% (SDS-PAGE)
• Form/Format: Liquid. In 20mM HEPES, pH8.0, containing 50mM sodium chloride and 1mM DTT

• Source Note: Human pro-ISG15 is fused at the N-terminus to a His-tag
• Preparation and Storage: For long term storage, store at -80 degree C; Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80 degree C; Shipping: Shipped on Dry Ice

Related Product Information for pro-ISG15 recombinant protein

ISG15 is an interferon (IFN)-alpha/beta-induced ubiquitin-like protein, also known as ubiquitin cross-reactive protein (UCRP), that is conjugated to cellular proteins during innate immune responses to bacterial and viral infections, including HIV. Although the biochemical consequences of ISG15 conjugation and the fate of the conjugated proteins are not currently known, it does not appear that ISG15 targets proteins for proteasomal degradation. ISG15 contains two ubiquitin homology domains in tandem and shows ~30% identity to ubiquitin. ISG15 is synthesized as a 17kDa pro-form (-GTEPGGRS C-terminal extension), which is subsequently cleaved to expose di-glycine residues at the carboxyl terminus, essential for subsequent conjugation to target proteins. Conjugation of ISG15 to cellular proteins occurs in a parallel but distinct mechanism to that of ubiquitin. The E1 activating enzyme for ISG15, is a single-subunit, ATP dependent enzyme, also known as Ube1L, while UbcH8, a member of the ubiquitin E2-conjugating enzyme family, has been identified as a major E2 enzyme for the ISGylation pathway, interacting with Ube1L to facilitate target protein ISGylation. BML-UW9230 is a recombinant protein produced in E Coli corresponding to the human pro-ISG15 sequence (Accession number: P05161) encompassing an N-terminal His₆ tag within a leader sequence.

Product Categories/Family for pro-ISG15 recombinant protein

Cellular Analysis; Stem Cells; Proteostasis; Protein Synthesis & Folding; Posttranslational Modifications; Ubiquitin & UBL Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 4826774
• NCBI GeneID: 9636
• NCBI Accession #: NP_005092.1
• NCBI GenBank Nucleotide #: NM_005101.3
• UniProt Accession #: P05161; Q5SVA4; Q7Z2G2; Q96GF0
• Molecular Weight: 17,888 Da
• NCBI Official Full Name: ubiquitin-like protein ISG15
• NCBI Official Synonym Full Names: ISG15 ubiquitin-like modifier
• NCBI Official Symbol: ISG15
• NCBI Official Synonym Symbols: G1P2; IP17; UCRP; IFI15; hUCRP
• NCBI Protein Information: ubiquitin-like protein ISG15; ubiquitin cross-reactive protein; interferon-stimulated protein, 15 kDa; interferon-induced 17-kDa/15-kDa protein; interferon, alpha-inducible protein (clone IFI-15K)
• UniProt Protein Name: Ubiquitin-like protein ISG15
• UniProt Gene Name: ISG15
• UniProt Synonym Gene Names: G1P2; UCRP; IP17; hUCRP
• UniProt Entry Name: ISG15_HUMAN

NCBI Description

The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012]

Uniprot Description

ISG15: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interaction with influenza B NS1 protein inhibits this conjugation. By type I interferons. Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: extracellular region; cytosol

Molecular Function: protein tag; protein binding

Biological Process: modification-dependent protein catabolic process; ISG15-protein conjugation; viral reproduction; negative regulation of viral genome replication; regulation of interferon-gamma production; cytokine and chemokine mediated signaling pathway; positive regulation of erythrocyte differentiation; defense response to bacterium; innate immune response; negative regulation of protein ubiquitination; defense response to virus; negative regulation of interferon type I production

Disease: Immunodeficiency 38