Insulin Receptor Substrate 1 ELISA Kit | IRS1 elisa kit

Rat IRS1 (Insulin Receptor Substrate 1) ELISA Kit

Cat. Num. AAA2533538

• Gene Names: IRS1; HIRS-1
• Reactivity: Rat
• Synonyms: Insulin Receptor Substrate 1; Rat IRS1 (Insulin Receptor Substrate 1) ELISA Kit; IRS1 elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinantRatIRS1. No significant cross-reactivity or interference between RatIRS1 and analogues was observed.
• Sequence Length: 1242

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IRS1 elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatIRS1concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IRS1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IRS1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IRS1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IRS1.You can calculate the concentration of IRS1in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5031805
• NCBI GeneID: 3667
• NCBI Accession #: NP_005535.1
• NCBI GenBank Nucleotide #: NM_005544.2
• Molecular Weight: 131,591 Da
• NCBI Official Full Name: insulin receptor substrate 1
• NCBI Official Synonym Full Names: insulin receptor substrate 1
• NCBI Official Symbol: IRS1
• NCBI Official Synonym Symbols: HIRS-1
• NCBI Protein Information: insulin receptor substrate 1
• UniProt Protein Name: Insulin receptor substrate 1
• Protein Family: Isoleucine--tRNA ligase
• UniProt Gene Name: IRS1
• UniProt Synonym Gene Names: IRS-1
• UniProt Entry Name: IRS1_HUMAN

NCBI Description

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]

Uniprot Description

IRS1: an adaptor protein that is one of the major substrates of the insulin receptor kinase. Contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain-containing proteins including phosphatidylinositol 3-kinase p85 subunit and GRB-2. Phosphorylated on ser/thr residues by kinases including JNK and IKK.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2q36

Cellular Component: caveola; cytoplasm; cytosol; intracellular membrane-bound organelle; nucleus; plasma membrane

Molecular Function: insulin receptor binding; insulin-like growth factor receptor binding; phosphoinositide 3-kinase binding; protein binding; protein kinase C binding; SH2 domain binding; signal transducer activity; transmembrane receptor protein tyrosine kinase adaptor protein activity

Biological Process: activation of MAPKK activity; axon guidance; cellular response to insulin stimulus; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; glucose homeostasis; innate immune response; insulin receptor signaling pathway; insulin-like growth factor receptor signaling pathway; lipid catabolic process; mammary gland development; MAPKKK cascade; negative regulation of insulin receptor signaling pathway; negative regulation of insulin secretion; nerve growth factor receptor signaling pathway; phosphoinositide 3-kinase cascade; phosphoinositide-mediated signaling; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of fatty acid beta-oxidation; positive regulation of glucose import; positive regulation of glycogen biosynthetic process; positive regulation of insulin receptor signaling pathway; positive regulation of mesenchymal cell proliferation; positive regulation of phosphoinositide 3-kinase activity; protein kinase B signaling cascade; Ras protein signal transduction; regulation of gene expression; response to insulin stimulus; response to peptide hormone stimulus; signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway

Disease: Diabetes Mellitus, Noninsulin-dependent