IQ Motif Containing GTPase Activating Protein 1 Recombinant Protein | IQGAP1 recombinant protein

Recombinant IQ Motif Containing GTPase Activating Protein 1 (IQGAP1)

Cat. Num. AAA2029521

• Gene Names: IQGAP1; SAR1; p195; HUMORFA01
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: IQ Motif Containing GTPase Activating Protein 1; Recombinant IQ Motif Containing GTPase Activating Protein 1 (IQGAP1); IQGAP1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; QDKFN VDEYSDLVTL TKPVIYISIGEIINTHTLLL DHQDAIAPEH NDPIHELLDD LGEVPTIESL IGESCGNSNDPNKEALAKTE VSLTLTNKFD VPGDENAEMD ARTILLNTKR LIVDVIRFQPGETLTEILET PATNEQEAEH QRAMQRRAIR DAKTPDKMKK SKPMKEDNNLSLQEKKEKIQ TGLKKLTELG TVDPKNRYQE LINDIAKDIR NQRRYRQRRKAELVKLQQTY SALNSKATFY GEQVDYYKSY IKTCLDNLAS KGKVSKKPREMKGKKSKKIS LKYTAARLHE KGVLLEIEDL QANQFKNVIF EIGPTEEVGDFEVKAKFMGV QMETFMLHYQ DLLQLQYEGV AVMKLFDRAK VNVNLLIFLLNKKFYGK
• Sequence Length: 1657

• Applicable Applications for IQGAP1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 47.5kDa
• Accurate Molecular Mass (KD): 58kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Gln1276~Lys1657
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 156230796
• NCBI GeneID: 8826
• Molecular Weight: 189,252 Da
• NCBI Official Full Name: IQ motif containing GTPase activating protein 1
• NCBI Official Synonym Full Names: IQ motif containing GTPase activating protein 1
• NCBI Official Symbol: IQGAP1
• NCBI Official Synonym Symbols: SAR1; p195; HUMORFA01
• NCBI Protein Information: ras GTPase-activating-like protein IQGAP1
• UniProt Protein Name: Ras GTPase-activating-like protein IQGAP1
• Protein Family: Ras GTPase-activating-like protein
• UniProt Gene Name: IQGAP1
• UniProt Synonym Gene Names: KIAA0051
• UniProt Entry Name: IQGA1_HUMAN

NCBI Description

This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq, Jul 2008]

Uniprot Description

IQGAP1: Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth. Interacts with CDC42; the interaction is demonstrated with IQGAP1 in GTP-bound and in nucleotide-free state. Interacts with RAC1. Does not interact with RHOA. Interacts with TSG101. Interacts with PAK6. Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas.

Protein type: GAPs, Ras; Motility/polarity/chemotaxis; GAPs

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: microtubule; neuron projection; focal adhesion; leading edge; actin filament; actin cytoskeleton; nucleoplasm; microtubule cytoskeleton; extrinsic to internal side of plasma membrane; growth cone; axon; cytoplasm; plasma membrane; midbody; cell junction; lateral plasma membrane

Molecular Function: calmodulin binding; protein serine/threonine kinase activator activity; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; Rac GTPase binding; protein complex binding; calcium ion binding; protein kinase binding; protein phosphatase binding; GTPase activator activity; GTPase inhibitor activity

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; positive regulation of protein kinase activity; negative regulation of dephosphorylation; small GTPase mediated signal transduction; negative regulation of catalytic activity; energy reserve metabolic process; platelet-derived growth factor receptor signaling pathway; signal transduction; regulation of insulin secretion; positive regulation of GTPase activity; regulation of cytokine production