Typical Testing Data/Standard Curve (for reference only)
Porcine Chemokine interferon-inducible protein 10 ELISA Kit | IP-10 elisa kit
Porcine Chemokine interferon-inducible protein 10 ELISA Kit
Gene Names
Cxcl10; C7; IP10; CRG-2; INP10; IP-10; Ifi10; mob-1; Scyb10; gIP-10
Reactivity
Porcine
Synonyms
Chemokine interferon-inducible protein 10; Porcine Chemokine interferon-inducible protein 10 ELISA Kit; IP-10 elisa kit
Reactivity
Porcine
Specificity
This assay has high sensitivity and excellent specificity for detection of IP-10. No significant cross-reactivity or interference between IP-10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IP-10 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IP-10 elisa kit
Intended Uses: This IP-10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine IP-10. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: IP-10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IP-10 antibody and an IP-10-HRP conjugate. The assay sample and buffer are incubated together with IP-10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IP-10 concentration since IP-10 from samples and IP-10-HRP conjugate compete for the anti-IP-10 antibody binding site. Since the number of sites is limited, as more sites are occupied by IP-10 from the sample, fewer sites are left to bind IP-10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IP-10 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: IP-10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IP-10 antibody and an IP-10-HRP conjugate. The assay sample and buffer are incubated together with IP-10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IP-10 concentration since IP-10 from samples and IP-10-HRP conjugate compete for the anti-IP-10 antibody binding site. Since the number of sites is limited, as more sites are occupied by IP-10 from the sample, fewer sites are left to bind IP-10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IP-10 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for IP-10 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
10,789 Da
NCBI Official Full Name
IP-10
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 10
NCBI Official Symbol
Cxcl10
NCBI Official Synonym Symbols
C7; IP10; CRG-2; INP10; IP-10; Ifi10; mob-1; Scyb10; gIP-10
NCBI Protein Information
C-X-C motif chemokine 10; gamma-IP10; interferon activated gene 10; small-inducible cytokine B10; interferon-gamma induced protein CRG-2; interferon-gamma-induced protein CRG-2; 10 kDa interferon gamma-induced protein; small inducible cytokine B subfamily (Cys-X-Cys), member 10
UniProt Protein Name
C-X-C motif chemokine 10
UniProt Gene Name
Cxcl10
UniProt Synonym Gene Names
Crg2; Ifi10; Inp10; Scyb10; Gamma-IP10; IP-10
UniProt Entry Name
CXL10_MOUSE
Uniprot Description
CXCL10: Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. Belongs to the intercrine alpha (chemokine CxC) family.
Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Chemokine
Cellular Component: extracellular space; cell; extracellular region; external side of plasma membrane
Molecular Function: heparin binding; CXCR3 chemokine receptor binding; chemokine activity; cytokine activity
Biological Process: positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of cAMP metabolic process; chemotaxis; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; positive regulation of cell proliferation; immune response; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of myoblast differentiation; inflammatory response; defense response to virus; positive regulation of cell migration
Research Articles on IP-10
Similar Products
Product Notes
The Porcine IP-10 cxcl10 (Catalog #AAA739741) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA739741 ELISA Kit recognizes Porcine IP-10. It is sometimes possible for the material contained within the vial of "Chemokine interferon-inducible protein 10, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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