ELISA (EIA)
(Bovine Interleukin-5 ELISARecombinant Bovine Interleukin-5 detected using Rabbit anti Bovine Interleukin-5 as the capture reagent and Rabbit anti Bovine Interleukin-5:Biotin as the detection reagent followed by Streptavidin:HRP.)
INTERLEUKIN-5 Recombinant Protein | IL-5 recombinant protein
RECOMBINANT BOVINE INTERLEUKIN-5
Reactivity
Bovine
Applications
ELISA, Western Blot
Purity
>95% by SDS PAGE analysis
Synonyms
INTERLEUKIN-5; RECOMBINANT BOVINE INTERLEUKIN-5; IL-5; IL-5 recombinant protein
Reactivity
Bovine
Specificity
IL-5
Purity/Purification
>95% by SDS PAGE analysis
Form/Format
Purified recombinant protein, lyophilized. Phosphate buffered saline
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Applicable Applications for IL-5 recombinant protein
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: This reagent may be used as a standard in a sandwich ELISA assay in conjunction with as a coating antibody and as a detection antibody.
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Species Cross-Reactivity
Goat, Sheep
Preservative Stabilizers
10% Trehalose
Reconstitution
Reconstitute with 20ul sterile PBS containing 0.1% carrier proteinCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.
Preparation and Storage
Prior to reconstitution store at -20 degree C.
After reconstitution store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
After reconstitution store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
ELISA (EIA)
(Bovine Interleukin-5 ELISARecombinant Bovine Interleukin-5 detected using Rabbit anti Bovine Interleukin-5 as the capture reagent and Rabbit anti Bovine Interleukin-5:Biotin as the detection reagent followed by Streptavidin:HRP.)
ELISA (EIA)
(Bovine Interleukin-5 ELISARecombinant Bovine Interleukin-5 detected using Rabbit anti Bovine Interleukin-5 as the capture reagent and Rabbit anti Bovine Interleukin-5:Biotin as the detection reagent followed by Streptavidin:HRP.)
Related Product Information for IL-5 recombinant protein
Recombinant Bovine Interleukin-5 (IL-5) is a recombinant protein corresponding to aa 22-134 of native bovine IL-5.
IL-5 is a regulatory cytokine that induces terminal differentiation of late-developing B cells to immunoglobulin secreting cells.
There is >85% identity with feline, bovine, ovine, canine, and human IL-5 sequences at the nucleotide and protein level.
IL-5 is a regulatory cytokine that induces terminal differentiation of late-developing B cells to immunoglobulin secreting cells.
There is >85% identity with feline, bovine, ovine, canine, and human IL-5 sequences at the nucleotide and protein level.
Similar Products
Product Notes
The IL-5 (Catalog #AAA226044) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. The RECOMBINANT BOVINE INTERLEUKIN-5 reacts with Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's INTERLEUKIN-5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: This reagent may be used as a standard in a sandwich ELISA assay in conjunction with as a coating antibody and as a detection antibody. Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the IL-5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "INTERLEUKIN-5, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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