PLGF1 Active Protein | Pgf active protein
PLGF1, Recombinant, Human (Placental Growth Factor 1)
Gene Names
Pgf; PIGF; Plgf; AI854365
Purity
Highly Purified90% as determined by:
Synonyms
PLGF1; Recombinant; Human (Placental Growth Factor 1); Pgf active protein
Host
Insect cells
Purity/Purification
Highly Purified
90% as determined by:
90% as determined by:
Form/Format
Supplied as a lyophilized powder containing BSA.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Pro-Ala-Val.
Biological Activity
Recombinant Human PLGF-1 is fully biologically active when compared to standard. The ED50 as determined by its ability to bind to immobilized rh-sFlt-1 in a functional ELISA. Recombinant human PlGF-1 can bind to immobilized rh-sFlt-1 (100 ng/well) with a linear range at 0.5 - 10 ng/mL.
Dimers and Aggregates
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin Level
0.1ng/ug (IEU/ug) of Recombinant PLGF-1.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a standard solution of PLGF-1 as a Reference Standard.
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a standard solution of PLGF-1 as a Reference Standard.
Solubility
It is recommended to reconstitute the lyophilized Recombinant PLGF-1 in sterile 20mM acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Preparation and Storage
-20 degree C
Stability: Lyophilized Human PLGF-1 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant Human PLGF-1 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Stability: Lyophilized Human PLGF-1 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant Human PLGF-1 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Related Product Information for Pgf active protein
Placenta growth factor (PlGF), a homodimeric glycoprotein that is homologous to vascular endothelial growth factor (VEGF), is mitogenic to endothelial cells and protects trophoblast from apoptosis. Alternative splicing of mature mRNA gives rise to three known isoforms of PlGF. PlGF is expressed by human trophoblast during normal pregnancy, however, it is not known which isoforms are produced.
Recombinant Human Placenta Growth Factor-1(PIGF/PGF) produced in insect cells is a homodimer, glycosylated polypeptide chain containing 2 x 131 amino acids and having a total molecular mass of 29.7kD. Recombinant Human PLGF-1 is purified by proprietary chromatographic techniques
Recombinant Human Placenta Growth Factor-1(PIGF/PGF) produced in insect cells is a homodimer, glycosylated polypeptide chain containing 2 x 131 amino acids and having a total molecular mass of 29.7kD. Recombinant Human PLGF-1 is purified by proprietary chromatographic techniques
Product Categories/Family for Pgf active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29.7kD
NCBI Official Full Name
placenta growth factor isoform 2
NCBI Official Synonym Full Names
placental growth factor
NCBI Official Symbol
Pgf
NCBI Official Synonym Symbols
PIGF; Plgf; AI854365
NCBI Protein Information
placenta growth factor
UniProt Protein Name
Placenta growth factor
Protein Family
UniProt Gene Name
Pgf
UniProt Synonym Gene Names
Plgf; PlGF
UniProt Entry Name
PLGF_MOUSE
Uniprot Description
Function: Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Also promotes cell tumor growth
By similarity.
Subunit structure: Antiparallel homodimer; disulfide-linked. Also found as heterodimer with VEGFA/VEGF
By similarity.
Subcellular location: Secreted
By similarity.
Sequence similarities: Belongs to the PDGF/VEGF growth factor family.
Research Articles on Pgf
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Product Notes
The Pgf pgf (Catalog #AAA650710) is an Active Protein produced from Insect cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Pr o-Ala-Val. It is sometimes possible for the material contained within the vial of "PLGF1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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