CETP active protein

Active Recombinant Human CETP

Cat. Num. AAA844442

• Gene Names: CETP; BPIFF; HDLCQ10
• Purity: > 90% by SDS-PAGE
• Synonyms: CETP; Active Recombinant Human CETP; BPIFF; Cholesteryl ester transfer protein; HDLCQ10; Lipid transfer protein I; CETP active protein

• Host: Insect Cells
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Lyophilized from a Buffer containing 10 mM Tris and 150 mM NaCl.
• Sequence Length: 493

• Application Notes: Active CETP is useful as standard in CETP activity assay, studying CETP regulation, determining target substrates, screening CETP inhibitors, performing structure-activity relationship (SAR) studies, etc. We recommend using 5-10 g protein per assay for analyzing CETP activity.
• Reconstitution: Reconstitute in sterile water at 2 mg/ml.
• Biological Activity: The CETP Activity Assay uses a donor molecule containing a fluorescent self-quenched neutral lipid that is transferred to an acceptor molecule in the presence of CETP. CETPmediated transfer of the fluorescent neutral lipid to the acceptor molecule results in an increase in fluorescence (Ex/Em: 465/535 nm).
• CETP Activity Assay: The CETP activity was detected with CETP Activity Assay Kit with 8ug of protein CETP in the buffer 10 mM Tris, pH 7.5, 150 mM NaCl, 2 mM EDTA. The fresh rabbit serum (3 ul) and non-CETP background were used as positive and negative controls, respectively.
• Preparation and Storage: Stable for 1 year at -20°C. ; Avoid multiple freeze/thaw cycles as activity may decrease.

Testing Data

Related Product Information for CETP active protein

Cholesteryl ester transfer protein (CETP), also called plasma lipid transfer protein, is a plasma protein that facilitates the transport of cholesteryl esters and triglycerides between the lipoproteins. It collects triglycerides from very-low-density (VLDL) or low-density lipoproteins (LDL) and exchanges them for cholesteryl esters from high-density lipoproteins (HDL), and vice versa. Inhibition of CETP has been proposed as a strategy to raise HDL cholesterol levels, a potential an approach to retarding atherogenesis and a strategy to reduce cardiovascular risk.

Product Categories/Family for CETP active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z) Proteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 169636439
• NCBI GeneID: 1071
• NCBI Accession #: NP_000069.2
• NCBI GenBank Nucleotide #: NM_000078.2
• UniProt Accession #: P11597; Q13987; Q13988; Q53YZ1
• Molecular Weight: 55 kDa
• NCBI Official Full Name: cholesteryl ester transfer protein isoform 1
• NCBI Official Synonym Full Names: cholesteryl ester transfer protein, plasma
• NCBI Official Symbol: CETP
• NCBI Official Synonym Symbols: BPIFF; HDLCQ10
• NCBI Protein Information: cholesteryl ester transfer protein
• UniProt Protein Name: Cholesteryl ester transfer protein
• Protein Family: Cholesteryl ester transfer protein
• UniProt Gene Name: CETP
• UniProt Entry Name: CETP_HUMAN

NCBI Description

The protein encoded by this gene is found in plasma, where it is involved in the transfer of cholesteryl ester from high density lipoprotein (HDL) to other lipoproteins. Defects in this gene are a cause of hyperalphalipoproteinemia 1 (HALP1). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Uniprot Description

CETP: Involved in the transfer of insoluble cholesteryl esters in the reverse transport of cholesterol. Defects in CETP are the cause of hyperalphalipoproteinemia type 1 (HALP1). Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL). Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Secreted, signal peptide; Lipid-binding; Secreted

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: extracellular space; extracellular region; vesicle

Molecular Function: lipid transporter activity; phospholipid transporter activity; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding; triglyceride binding

Biological Process: receptor-mediated endocytosis; cholesterol metabolic process; cholesterol transport; lipid homeostasis; lipoprotein metabolic process; lipid transport; phosphatidylcholine metabolic process; cholesterol homeostasis; reverse cholesterol transport; triacylglycerol metabolic process; phospholipid transport; triacylglycerol transport; transmembrane transport; phospholipid homeostasis

Disease: Hyperalphalipoproteinemia 1