Sheep Insulin ELISA Kit | INS elisa kit

Sheep Insulin ELISA Kit

Cat. Num. AAA742754

• Gene Names: INS; ILPR; IRDN; IDDM1; IDDM2; MODY10
• Reactivity: Sheep
• Synonyms: Insulin; Sheep Insulin ELISA Kit; INS elisa kit

• Reactivity: Sheep
• Specificity: This assay has high sensitivity and excellent specificity for detection of INS. No significant cross-reactivity or interference between INS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between INS and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for INS elisa kit

Intended Uses: This INS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep INS. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: INS ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-INS antibody and an INS-HRP conjugate. The assay sample and buffer are incubated together with INS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the INS concentration since INS from samples and INS-HRP conjugate compete for the anti-INS antibody binding site. Since the number of sites is limited, as more sites are occupied by INS from the sample, fewer sites are left to bind INS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INS concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 386828
• NCBI GeneID: 3630
• Molecular Weight: 21,537 Da
• NCBI Official Full Name: insulin
• NCBI Official Synonym Full Names: insulin
• NCBI Official Symbol: INS
• NCBI Official Synonym Symbols: ILPR; IRDN; IDDM1; IDDM2; MODY10
• NCBI Protein Information: insulin; proinsulin; preproinsulin; insulin-dependent diabetes mellitus 2
• UniProt Protein Name: Insulin
• Protein Family: Insulin
• UniProt Gene Name: INS
• UniProt Entry Name: INS_HUMAN

NCBI Description

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Uniprot Description

Insulin: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds. Belongs to the insulin family.

Protein type: Secreted; Hormone; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; Golgi lumen; endoplasmic reticulum lumen; extracellular region

Molecular Function: identical protein binding; insulin-like growth factor receptor binding; protein binding; protease binding; hormone activity; insulin receptor binding

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of vasodilation; glucose homeostasis; activation of NF-kappaB transcription factor; positive regulation of glucose import; negative regulation of protein oligomerization; regulation of protein localization; cell-cell signaling; negative regulation of gluconeogenesis; acute-phase response; regulation of transmembrane transporter activity; negative regulation of NAD(P)H oxidase activity; positive regulation of mitosis; activation of protein kinase B; positive regulation of nitric-oxide synthase activity; negative regulation of acute inflammatory response; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of cell growth; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of protein catabolic process; positive regulation of cell differentiation; regulation of amino acid metabolic process; negative regulation of glycogen catabolic process; wound healing; positive regulation of glycogen biosynthetic process; positive regulation of cellular protein metabolic process; glucose transport; regulation of protein secretion; positive regulation of MAPKKK cascade; regulation of transcription, DNA-dependent; negative regulation of vasodilation; negative regulation of lipid catabolic process; positive regulation of cell proliferation; negative regulation of proteolysis; negative regulation of protein secretion; MAPKKK cascade; alpha-beta T cell activation; negative regulation of fatty acid metabolic process; endocrine pancreas development; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; cellular protein metabolic process; fatty acid homeostasis; positive regulation of glycolysis; energy reserve metabolic process; insulin receptor signaling pathway; positive regulation of insulin receptor signaling pathway; regulation of insulin secretion; positive regulation of DNA replication; positive regulation of cytokine secretion; positive regulation of cell migration

Disease: Diabetes Mellitus, Insulin-dependent, 2; Diabetes Mellitus, Permanent Neonatal; Hyperproinsulinemia; Maturity-onset Diabetes Of The Young, Type 10