Chemical Structure
ON-01910, inhibitor
ON-01910
Purity
0.9937
Synonyms
ON-01910; Rigosertib; Estybon; inhibitor
Purity/Purification
0.9937
Form/Format
Solid
Solubility
DMSO: 88mg/mL (185.9mM)
Water: 87mg/mL (183.7mM)
Ethanol: <1mg/mL
(<1mg/ml refers to the product slightly soluble or insoluble)
Water: 87mg/mL (183.7mM)
Ethanol: <1mg/mL
(<1mg/ml refers to the product slightly soluble or insoluble)
SMILES
COc1c(cc(cc1)CS(=O)(=O)/C=C/c1c(cc(cc1OC)OC)OC)NCC(=O)[O-].[Na+]
Formula
C21H24NNaO8S
Cell Research
Cells are grown in either DMEM or RPMI supplemented with 10% fetal bovine serum and 1 unit/mL penicillin-streptomycin solution. Tumor cells are plated into six-well dishes at a density of 1 x 105cells/mL/well, and Rigosertib is added 24 hours later at various concentrations. Cell counts are determined from duplicate wells after 96-hour of treatment. The total number of viable cells is determined by trypan blue exclusio(Only for Reference)
Kinase Assay
In vitro enzyme assays for PLK1: Recombinant PLK1 (10ng) is incubated with different concentrations of Rigosertib in a 15uL reaction mixture (50mM HEPES, 10mM MgCl2, 1mM EDTA, 2mM Dithiothreitol, 0.01% NP-40 [pH 7.5]) for 30 min at room temperature. Kinase reactions are performed for 20 min at 30 degree C in a volume of 20uL (15uL enzyme + inhibitor, 2uL 1mM ATP), 2uL of gamma32P-ATP (40uCi), and 1uL of recombinant Cdc25C (100ng) or casein (1ug) substrates. Reactions are terminated by boiling for 2 min in 20uL of 2x Laemmli buffer. Phosphorylated substrates are separated by 18% SDS-PAGE. The gels are dried and exposed to X-ray film for 3-10 min.
CAS Number
592542-60-4
Preparation and Storage
Store at 0-4 degree C for short term (days to weeks), or -20 degree C for long term (months).
Chemical Structure
Chemical Structure
Related Product Information for ON-01910, inhibitor
Rigosertib, a non-ATP-competitive inhibitor of PLK1(IC50 = 9), exhibits 30-fold higher specificity activity over Plk2 and no effect on Plk3.
Product Categories/Family for ON-01910, inhibitor
References
Gumireddy K, et al. Cancer Cell, 2005, 7(3), 275-286.
Reddy MV, et al. J Med Chem, 2011, 54(18), 6254-6276.
Chapman CM, et al. Clin Cancer Res, 2012 18(7), 1979-1991.
Reddy MV, et al. J Med Chem, 2011, 54(18), 6254-6276.
Chapman CM, et al. Clin Cancer Res, 2012 18(7), 1979-1991.
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Product Notes
The ON-01910 (Catalog #AAA5764904) is an Inhibitor and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ON-01910, Inhibitor" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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