Structure
E-64d, inhibitor
E-64d
Purity
>=98%
Synonyms
E-64d; inhibitor
Purity/Purification
>=98%
Form/Format
E-64d is supplied as a crystalline solid.
Application Notes
A stock solution may be made by dissolving the E-64d in the solvent of choice. E-64d is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of E-64d in ethanol is approximately 10 mg/ml and approximately 30 mg/ml in DMSO and DMF.
E-64d is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, E-64d should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. E-64d has a solubility of approximately 0.5 mg/ml in a 1:1 solution of DMSO:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
E-64d is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, E-64d should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. E-64d has a solubility of approximately 0.5 mg/ml in a 1:1 solution of DMSO:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Molecular Formula
C17H30N2O5
Preparation and Storage
Store at -20 degree C, product is stable for at least two years.
Structure
Structure
Related Product Information for E-64d, inhibitor
Cysteine proteases are a class of enzymes containing an active-site cysteine residue that is important in protein degradation pathways. E-64d, a synthetic analog of E-64 and ethyl ester of E-64c, is an irreversible, membrane-permeable inhibitor of lysosomal and cytosolic cysteine proteases.E-64d inhibits calpain and the cysteine proteases cathepsins F, K, B, H, and L. By disrupting protease activity, E-64d, at concentrations between 20-200 uM, has been shown to arrest human epidermoid carcinoma A431 cells at mitotic metaphase.It also inhibits protease-resistant prion protein accumulation in scrapie-infected neuroblastoma cells with an IC50 value of 0.5 uM.
Similar Products
Product Notes
The E-64d (Catalog #AAA669386) is an Inhibitor and is intended for research purposes only. The product is available for immediate purchase. A stock solution may be made by dissolving the E-64d in the solvent of choice. E-64d is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of E-64d in ethanol is approximately 10 mg/ml and approximately 30 mg/ml in DMSO and DMF. E-64d is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, E-64d should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. E-64d has a solubility of approximately 0.5 mg/ml in a 1:1 solution of DMSO:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day. Researchers should empirically determine the suitability of the E-64d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E-64d, Inhibitor" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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