2-Iminobiotin hydrobromide, Inhibitor

2-Iminobiotin hydrobromide

Cat. Num. AAA5764918

• Synonyms: 2-Iminobiotin hydrobromide; Guanidinobiotin; inhibitor

• Form/Format: Solid

• Solubility: DMSO: 12.5mg/mL (51.37mM); (<1mg/ml refers to the product slightly soluble or insoluble)
• SMILES: [H][C@](CS[C@H]1CCCCC(O)=O)([C@]1([H])N2)NC2=N.Br
• Formula: C10H18BrN3O2S
• Cell Research: In vitro hypoxia was induced for 7 h in IMR-32 cell cultures by using our recently described system with minor modifications. Enzyme stock solutions (100x) of catalase and glucose oxidase were diluted in cell culture medium (DMEM/F12, 1% FCS; final concentration: 120 and 2 U/ml respectively). A rapid decrease of partial pressure of oxygen (pO2) to levels below 10 mmHg was achieved by adding the enzymes to glucose containing culture medium. Also a decline in glucose (<1 g/l) and pH (<7.0) was observed, resembling the clinical characteristics of hypoxic-ischemic injury in vivo. Hypoxic conditions were confirmed with a tissue oxygen pressure monitor. After the hypoxic insult, cells were washed twice with PBS and cultures were placed into an incubator at 33.5 degree C (hypothermia) employing culture medium with (i) solvent (citrate buffer 1%) or (ii) 2-IB at 10, 30, 50, 100, and 300 ng/ml. To determine the optimal "reperfusion" time, a time-interval curve investigating cell damage (LDH release) was performed. Analyses of LDH release, ROS generation, hydrogen peroxide release, metabolic activity, cell signaling, apoptosis-related protein expression/activity and expression analysis of 35 human apoptosis-related proteins were performed at different time points post-hypoxia[1]
• CAS Number: 76985-52-9
• Preparation and Storage: Store at 0-4 degree C for short term (days to weeks), or -20 degree C for long term (months).

Chemical Structure

Related Product Information for 2-Iminobiotin hydrobromide, inhibitor

2-Iminobiotin hydrobromide is a cyclic guanidino analog of biotin that acts as a reversible inhibitor of inducible nitric oxide synthase (iNOS) and neuronal NOS (nNOS; Ki of 21.8 and 37.5uM for mouse iNOS and rat nNOS, respectively).

Product Categories/Family for 2-Iminobiotin hydrobromide, inhibitor

Immunology/Inflammation; NOS

References

Zitta K, Peeters-Scholte C, Sommer L, et al. 2-Iminobiotin Superimposed on Hypothermia Protects Human Neuronal Cells from Hypoxia-Induced Cell Damage: An in Vitro Study[J]. Frontiers in Pharmacology, 2018, 8:971.
Sup S J, Green B G, Grant S K. 2-Iminobiotin Is an Inhibitor of Nitric Oxide Synthases[J]. Biochemical & Biophysical Research Communications, 1994, 204(2):0-968.