Influenza A H4N2 Hemagglutinin/HA Recombinant Protein | HA recombinant protein

Influenza A H4N2 (A/duck/Hunan/8-19/2009) Hemagglutinin/HA Protein (His Tag)

Cat. Num. AAA8118794

• Purity: >95 % as determined by SDS-PAGE.
• Synonyms: Influenza A H4N2 Hemagglutinin/HA; Influenza A H4N2 (A/duck/Hunan/8-19/2009) Hemagglutinin/HA Protein (His Tag); Harvey rat sarcoma viral oncogene homolog; HA recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH8.0, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 522aa
• Sequence: Met1-Asp527

• Species: H4N2
• Tag: C-His
• Endotoxin: <1.0EU per ug protein as determined by the LAL method
• Predicted N Terminal: Gln17
• Protein Construction: |A DNA sequence encoding the Influenza A virus (A/duck/Hunan/8-19/2009(H4N2)) hemagglutinin (ADN78261.1) (Met1-Asp527), termed as HA, was expressed with a polyhistidine tag at the C-terminus
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Related Product Information for HA recombinant protein

The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

References

White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
Donald J. Benton, Influenza hemagglutinin membrane anchor, PNAS, 2018

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant Influenza A virus (A/duck/Hunan/8-19/2009 (H4N2)) hemagglutinin consists of 522 amino acids and predicts a molecular mass of 58.8kDa.
• Protein Family: Protein