Lung Tumor Blot II Immunoblot
Human Lung Tumor Blot II
Applications
Western Blot
Synonyms
Lung Tumor Blot II; Human Lung Tumor Blot II; Lung Tumor Blot II immunoblot
Applicable Applications for Lung Tumor Blot II immunoblot
Western Blot (WB)
Application Notes
For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
Species
Human
Quantity Loaded Per Lane
15 ug
By Sample Identification
Tumor
Samples Loaded
Lanes: 1, protein markers; 2, A549; 3, small cell carcinoma; 4, matched normal lung tissue; 5, non-small cell carcinoma; 6, matched normal lung tissue; 7, squamous cell carcinoma; and 8, matched normal lung tissue.
Note
Lysate lane order may vary; please consult accompanying shipment paperwork.
Preparation and Storage
Store at 4 degree C. The blot can be reused after stripping.
Related Product Information for Lung Tumor Blot II immunoblot
The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries the cell line A549 (human lung carcinoma), and 3 different human lung tumor tissue lysates and matched normal lysates (from non-tumor lung tissue from near the tumor) with 15 ug of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The lung tumor tissues included in the blot are 1 small cell carcinoma, 1 non-small cell carcinoma, and 1 squamous cell carcinoma.
Similar Products
Product Notes
The Lung Tumor Blot II (Catalog #AAA153743) is an Immunoblot and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Lung Tumor Blot II can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature. Researchers should empirically determine the suitability of the Lung Tumor Blot II for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lung Tumor Blot II, Immunoblot" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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