ILF3 / Interleukin Enhancer-Binding Factor 3 Recombinant Protein | ILF3 recombinant protein

Human ILF3 / Interleukin Enhancer-Binding Factor 3 Recombinant Protein

• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: ILF3 / Interleukin Enhancer-Binding Factor 3; Human ILF3 / Interleukin Enhancer-Binding Factor 3 Recombinant Protein; Interleukin enhancer-binding factor 3; Double-stranded RNA-binding protein 76; DRBP76; M-phase phosphoprotein 4; MPP4; Nuclear factor associated with dsRNA; NFAR; Nuclear factor of activated T-cells 90 kDa; NF-AT-90; Translational control protein 80; TCP80; DRBF; MPHOSPH4; NF90; ILF3 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 894

• Applicable Applications for ILF3 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with FUS and SMN proteins and with PRMT1. Forms a complex with ILF2. Can also bind to PRKDC/XRCC7: this may stabilize the interaction of PRKDC/XRCC7 and the heterodimeric complex of XRCC6/KU70 and XRCC5/KU80. Forms a heteromeric complex with ZNF346 and ILF3. Found in a nuclear export complex with XPO5, ILF3, Ran and double-stranded RNA or double-stranded minihelix VA1 RNA. Found in a nuclear export complex with XPO5, RAN, ILF3, ZNF346 and double-stranded RNA. Interacts with XPO5 and ZNF346. Forms a complex with ILF2, YLPM1, KHDRBS1, RBMX, NCOA5 and PPP1CA. Interacts with AGO1 and AGO2.
• Entry Function: May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N-methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. The phosphorylated form at Thr-188 and Thr-315, in concert with EIF2AK2/PKR can inhibit vesicular stomatitis virus (VSV) replication.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 62512150
• UniProt Accession #: Q12906
• NCBI Official Full Name: Interleukin enhancer-binding factor 3
• UniProt Protein Name: Interleukin enhancer-binding factor 3
• Protein Family: Interleukin enhancer-binding factor
• UniProt Gene Name: ILF3
• UniProt Synonym Gene Names: DRBF; MPHOSPH4; NF90; DRBP76; MPP4; NFAR; NF-AT-90; TCP80
• UniProt Entry Name: ILF3_HUMAN

Uniprot Description

NFAT90: a double-stranded RNA (dsRNA)-binding protein implicated in the regulation of gene expression . Phosphorylated in a double-stranded RNA-dependent manner possibly by PKR. Five different splice-variant isoforms have been described.

Protein type: RNA-binding; Transcription factor; DNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; membrane; mitochondrion; cytoplasm; nucleolus; ribonucleoprotein complex; nucleus

Molecular Function: protein binding; DNA binding; RNA binding; double-stranded RNA binding

Biological Process: transcription, DNA-dependent; negative regulation of viral genome replication; positive regulation of transcription, DNA-dependent; negative regulation of translation; negative regulation of transcription, DNA-dependent; defense response to virus; protein amino acid phosphorylation