Interleukin 6 Receptor (IL6R) Recombinant Protein | IL6R recombinant protein

Recombinant Interleukin 6 Receptor (IL6R)

Cat. Num. AAA2009653

• Gene Names: Il6r; IL6R1; Il6ra
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Interleukin 6 Receptor (IL6R); Recombinant Interleukin 6 Receptor (IL6R); IL6R recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-LRLKKK WKSQAEKESK TTSPPPYPLG PLKPTFLLVP LLTPSGSHNS SGTDNTGSHS CLGVRDPQCP NDNSNRDYLF PR
• Sequence Length: 462

• Applicable Applications for IL6R recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Leu385~Arg462 (Accession # P22273) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 729832
• NCBI GeneID: 24499
• UniProt Accession #: P22273
• Molecular Weight: 10.1kDa
• NCBI Official Full Name: Interleukin-6 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 6 receptor
• NCBI Official Symbol: Il6r
• NCBI Official Synonym Symbols: IL6R1; Il6ra
• NCBI Protein Information: interleukin-6 receptor subunit alpha; IL-6RA; IL-6R 1; IL-6R-alpha; IL-6R subunit alpha; IL-6 receptor subunit alpha; interleukin 6 receptor, alpha
• UniProt Protein Name: Interleukin-6 receptor subunit alpha
• Protein Family: Interleukin-6 receptor
• UniProt Gene Name: Il6r
• UniProt Synonym Gene Names: Il6ra; IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA
• UniProt Entry Name: IL6RA_RAT

NCBI Description

alpha subunit of the interleukin 6 receptor complex [RGD, Feb 2006]

Uniprot Description

Function: Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.

Subunit structure: Hexamer of two molecules each of IL6, IL6R and IL6ST

By similarity.

Subcellular location: Membrane; Single-pass type I membrane protein.

Domain: The two fibronectin type-III-like domains contained in the C-terminal part form together a cytokine-binding domain.The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

Sequence similarities: Belongs to the type I cytokine receptor family. Type 3 subfamily.Contains 1 fibronectin type-III domain.Contains 1 Ig-like C2-type (immunoglobulin-like) domain.