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SDS-Page (Figure 1. Human IL6 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)

IL6 recombinant protein

Recombinant human IL6 protein with C-terminal mouse Fc and 6xHis tag

Gene Names
IL6; HGF; HSF; BSF2; IL-6; IFNB2
Applications
Western Blot, ELISA
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Synonyms
IL6; Recombinant human IL6 protein with C-terminal mouse Fc and 6xHis tag; Human IL6 Protein; mFc-His Tag; Interleukin-6; BSF2; HSF; IFNB2; IL6 recombinant protein
Ordering
For Research Use Only!
Host
HEK293 cells
Purity/Purification
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5%-39% trehalose is added as protectants before lyophilization.
Sequence
IL6(Val30-Met212)+mFc(Pro99-Lys330)
Applicable Applications for IL6 recombinant protein
Western Blot (WB), ELISA (EIA)
Source
Human
Conjugation
C-Mouse Fc and 6xHis tag
Reconstitution
Reconstitute with deionized water
Preparation and Storage
Store at -80 degree C for 12 months (Avoid repeated freezing and thawing)
Shipping: Blue Ice

SDS-Page

(Figure 1. Human IL6 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)

SDS-Page (Figure 1. Human IL6 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)

ELISA (EIA)

(Figure 2. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human IL6, mFc-His tagged protein can bind Anti-IL6 Neutralizing antibody in a linear range of 7.81-386.8 ng/ml.)

ELISA (EIA) (Figure 2. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human IL6, mFc-His tagged protein can bind Anti-IL6 Neutralizing antibody in a linear range of 7.81-386.8 ng/ml.)

ELISA (EIA)

(Figure 3. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human IL6R, His tagged protein can bind Human IL6, mFc-His tagged protein in a linear range of 0.512-40000 ng/ml.)

ELISA (EIA) (Figure 3. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human IL6R, His tagged protein can bind Human IL6, mFc-His tagged protein in a linear range of 0.512-40000 ng/ml.)
Related Product Information for IL6 recombinant protein
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
212
NCBI Official Full Name
Interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
IL6
NCBI Official Synonym Symbols
HGF; HSF; BSF2; IL-6; IFNB2
NCBI Protein Information
interleukin-6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; interferon, beta 2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor
UniProt Protein Name
Interleukin-6
Protein Family
UniProt Gene Name
IL6
UniProt Synonym Gene Names
IFNB2; IL-6; BSF-2; CDF; IFN-beta-2
UniProt Entry Name
IL6_HUMAN

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Product Notes

The IL6 il6 (Catalog #AAA188088) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IL6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the IL6 il6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: IL6(Val30- Met212)+mF c(Pro99-Ly s330). It is sometimes possible for the material contained within the vial of "IL6, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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