Interleukin-6 Recombinant Protein | IL-6 recombinant protein

Mouse Interleukin-6 Recombinant

Cat. Num. AAA553163

• Purity: >97%, as determined by SDS-PAGE and HPLC
• Synonyms: Interleukin-6; Mouse Interleukin-6 Recombinant; il-6; il6; interleukin-6; B-cell stimulatory factor; bsf; IL-6 recombinant protein

• Host: E Coli
• Purity/Purification: >97%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant Interleukin-6 was lyophilized from a 0.2 um filtered PBS solution.
• Sequence: MKFLSARDFH PVAFLGLMLV TTTAFPTSQV RRGDFTEDTT PNRPVYTTSQ VGGLITHVLW EIVEMRKELC NGNSDCMNND DALAENNLKL PEIQRNDGCY QTGYNQEICL LKISSGLLEY HSYLEYMKNN LKDNKKDKAR VLQRDTETLI HIFNQEVKDL HKIVLPTPIS NALLTDKLES QKEWLRTKTI QFILKSLEEF LKVTLRSTRQ T
• Sequence Length: 211

• Domain: four helical cytokine core
• Host Note: Optimized DNA sequence encoding Mouse Interleukin-6 mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native Mouse Interleukin-6, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 22 kDa. Recombinant IL-6 is a disulfide-linked homodimeric protein consisting of 188 amino acid residue subunits, and migrates as an approximately 22 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for IL-6 recombinant protein

IL6 is produced by many different cell types. The main sources in vivo are stimulated monocytes, fibroblasts, and endothelial cells. Macrophages, T-cells and B-lymphocytes, granulocytes, smooth muscle cells, eosinophils, chondrocytes, osteoblasts, mast cells, glial cells, and keratinocytes also produce IL6 after stimulation. IL6 is a member of a family of cytokines, which also includes LIF, CNTF, Oncostatin M, IL11, and CT- 1. All known members of the IL6 cytokine family induce hepatic expression of acute phase proteins. The IL6 receptor is expressed on T-cells, mitogen-activated B-cells, peripheral monocytes and some macrophage- and B-cell derived tumor cell types. It is not expressed in resting B-cells but in resting T- cells. The IL6 receptor is a strongly glycosylated protein of 80 kDa and a length of 449 amino acids. It has been designated CD126.

Product Categories/Family for IL-6 recombinant protein

Cytokines

References

• IL-6 protects against hyperoxia-induced mitochondrial damage via bcl-2–induced bak interactions with mitofusions .Am. J. Respir. Cell Mol. Biol., Oct 2009; 41: 385 - 396.
• long-term safety and efficacy of tocilizumab, an anti-IL-6 receptor monoclonal antibody, in monotherapy, in patients with rheumatoid arthritis (the stream study) : evidence of safety and efficacy in a 5-year extension study .Ann Rheum Dis, Oct 2009; 68: 1580 - 1584.
• the second bovine β-galactoside-2,6-sialyltransferase (st6gal ii) : genomic organization and stimulation of its in vitro expression by IL-6 in bovine mammary epithelial cells .Glycobiology, Oct 2009; 19: 1082 - 1093.
• IL-6 -174g/c promoter polymorphism in acute appendicitis .Lab Med, Oct 2009; 40: 600 - 603.
• cell surface-bound il-1 is an upstream regulator of the senescence-associated IL-6/il-8 cytokine network .PNAS, Sep 2009; 10.1073/pnas.0905299106.