Interleukin-5 Recombinant Protein | IL5 recombinant protein
Mouse Interleukin-5 Recombinant
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
Interleukin-5; Mouse Interleukin-5 Recombinant; TRF; il5; il-5; interleukin-5; IL5 recombinant protein
Host
Insect Cells
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant Interleukin-5 was lyophilized from a 0.2 um filtered PBS solution.
Sequence
MRRMLLHLSV LTLSCVWATA MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG
Sequence Length
133
Domain
four helix cytokine core
Host Note
Optimized DNA sequence encoding Mouse Interleukin-5 mature chain was expressed in Insect Cells
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Native Mouse Interleukin-5, generated by the proteolytic removal of the signal peptide
and propeptide, the molecule has a calculated molecular mass of approximately 13 kDa.
Recombinant mouse IL-5 is a disulfide-linked homodimeric protein consisting of two 113 amino acid residue subunits, and migrates as an approximately 26 kDa protein under non-reducing conditions and as a 13 kDa protein under reducing conditions in SDS-PAGE.
and propeptide, the molecule has a calculated molecular mass of approximately 13 kDa.
Recombinant mouse IL-5 is a disulfide-linked homodimeric protein consisting of two 113 amino acid residue subunits, and migrates as an approximately 26 kDa protein under non-reducing conditions and as a 13 kDa protein under reducing conditions in SDS-PAGE.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Related Product Information for IL5 recombinant protein
Interleukin-5 is a specific hematopoietic growth factor that is responsible for the growth and differentiation of eosinophils. IL-5 promotes the growth of immature hematopoietic progenitor cells BFU-E while it causes differentiation of CFU-E the proliferation of which is inhibited by IL-5. IL-5 strongly stimulates the proliferation, cell activation, and differentiation of eosinophils. B-cells can be made responsive to IL5 by treatment with suboptimal doses of IL1.
Product Categories/Family for IL5 recombinant protein
References
• systemic overexpression of ifn- and IL-5 exacerbates early phase reaction and conjunctival eosinophilia, respectively, in experimental allergic conjunctivitis .Br J Ophthalmol, Aug 2009; 10.1136/bjo.2009.162594.
• regulation of myelopoiesis through syntenin-mediated modulation of IL-5 receptor output .Blood, Aug 2009; 10.1182/blood-2009-03-208850.
• il-33 induces antigen-specific IL-5+ t cells and promotes allergic-induced airway inflammation independent of il-4 .J. Immunol., Oct 2008; 181: 4780 - 4790.
• in vitro study of interleukin-5 (IL-5) in human eosinophilic chronic rhinosinusitis cell culture .In Vivo, Sep 2008; 22: 549 - 556.
• separate endocytic pathways regulate IL-5 receptor internalization and signaling .J. Leukoc. Biol., Aug 2008; 84: 499 - 509.
• regulation of myelopoiesis through syntenin-mediated modulation of IL-5 receptor output .Blood, Aug 2009; 10.1182/blood-2009-03-208850.
• il-33 induces antigen-specific IL-5+ t cells and promotes allergic-induced airway inflammation independent of il-4 .J. Immunol., Oct 2008; 181: 4780 - 4790.
• in vitro study of interleukin-5 (IL-5) in human eosinophilic chronic rhinosinusitis cell culture .In Vivo, Sep 2008; 22: 549 - 556.
• separate endocytic pathways regulate IL-5 receptor internalization and signaling .J. Leukoc. Biol., Aug 2008; 84: 499 - 509.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,410 Da
NCBI Official Full Name
interleukin-5
UniProt Protein Name
Interleukin-5
Protein Family
UniProt Gene Name
Il5
UniProt Synonym Gene Names
Il-5; IL-5; BCGF-II; TRF
UniProt Entry Name
IL5_MOUSE
Uniprot Description
IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.
Protein type: Cytokine; Secreted, signal peptide; Secreted
Cellular Component: extracellular space; extracellular region
Molecular Function: interleukin-5 receptor binding; growth factor activity; cytokine activity
Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of JAK-STAT cascade; immune response; positive regulation of transcription factor activity; positive regulation of immunoglobulin secretion; positive regulation of eosinophil differentiation
Similar Products
Product Notes
The IL5 il5 (Catalog #AAA553280) is a Recombinant Protein produced from Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MRRMLLHLSV LTLSCVWATA MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG. It is sometimes possible for the material contained within the vial of "Interleukin-5, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
