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Western Blot (WB) ()

IL4 recombinant protein

IL4 protein (His tag)

Applications
ELISA, SDS-Page, Western Blot
Purity
> 90% pure
Synonyms
IL4; IL4 protein (His tag); IL-4 protein; IL 4 protein; B cell stimulatory factor 1 protein; BSF 1 protein; Binetrakin protein; Lymphocyte stimulatory factor 1 protein; Pitrakinra protein; Interleukin 4 protein; IL4 recombinant protein
Ordering
Host
Human
Purity/Purification
> 90% pure
Form/Format
Supplied in liquid form in PBS buffer
Sequence
HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATA  QQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS  
Sequence Length
153
Applicable Applications for IL4 recombinant protein
ELISA (EIA), SDS-PAGE, Western Blot (WB)
Source Note
His-fusion expressed in cell supernatent (soluble under denaturing conditions)
Protein Type
Recombinant
Biological Significance
IL4 participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
Expression System
E Coli
Tag/Conjugate
His tag
Preparation and Storage
Store at 4 degree C in a working aliquot for 1 week. For long term storage, aliquot and freeze at -20 to -80 degree C, avoid repeat freeze/thaw cycles

Western Blot (WB)

()

Western Blot (WB) ()
Related Product Information for IL4 recombinant protein
Purified recombinant IL4 protein (His tag)
Product Categories/Family for IL4 recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
Molecular Weight
20 kDa
NCBI Official Full Name
IL4, partial
Protein Family

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Product Notes

The IL4 (Catalog #AAA5304587) is a Recombinant Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IL4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), SDS-PAGE, Western Blot (WB). Researchers should empirically determine the suitability of the IL4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HKCDITLQEI IKTLNSLTEQ KTLCTELTVT DIFAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA  QQFHRHKQ LIRFLKRLDR NLWGLAGLNS CPVKEANQST LENFLERLKT IMREKYSKCS S  . It is sometimes possible for the material contained within the vial of "IL4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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