Human Interleukin-34 ELISA Kit | IL34 elisa kit

Human Interleukin-34 ELISA Kit

Cat. Num. AAA2887618

• Gene Names: IL34; IL-34; C16orf77
• Reactivity: Human
• Synonyms: Interleukin-34; Human Interleukin-34 ELISA Kit; IL-34; IL34; C16orf77; IL34 elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Interleukin-34,IL-34
• Sequence Length: 241

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 15.6-1000 pg/mL
• Sensitivity: <3.9 pg/mL
• Intra-assay Precision: <=3.3%
• Inter-assay Precision: <=5.5%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL34 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 289666750
• NCBI GeneID: 146433
• NCBI Accession #: NP_001166242.1
• NCBI GenBank Nucleotide #: NM_001172771.1
• UniProt Accession #: Q6ZMJ4; Q8N6L2; B2RC28; B2Z4A8; B2ZC70
• Molecular Weight: 27,354 Da
• NCBI Official Full Name: interleukin-34 isoform 2
• NCBI Official Synonym Full Names: interleukin 34
• NCBI Official Symbol: IL34
• NCBI Official Synonym Symbols: IL-34; C16orf77
• NCBI Protein Information: interleukin-34
• UniProt Protein Name: Interleukin-34
• Protein Family: Interleukin
• UniProt Gene Name: IL34
• UniProt Synonym Gene Names: C16orf77; IL-34
• UniProt Entry Name: IL34_HUMAN

NCBI Description

Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al., 2008 [PubMed 18467591]).[supplied by OMIM, May 2008]

Uniprot Description

IL34: Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Belongs to the IL-34 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: extracellular space

Molecular Function: cytokine activity; growth factor activity; macrophage colony stimulating factor receptor binding

Biological Process: inflammatory response; innate immune response; positive regulation of cell proliferation; positive regulation of macrophage differentiation; positive regulation of monocyte differentiation; positive regulation of protein amino acid phosphorylation