Human Interleukin-31 ELISA Kit | IL31RA elisa kit
Human Interleukin-31 receptor subunit alpha (IL31RA) ELISA Kit
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IL31RA in samples. An antibody specific for IL31RA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL31RA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL31RA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL31RA bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the type I cytokine receptor family. This receptor, with homology to gp130, is expressed on monocytes, and is involved in IL-31 signaling via activation of STAT-3 and STAT-5. It functions either as a monomer, or as part of a receptor complex with oncostatin M receptor (OSMR). Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]
Uniprot Description
IL31RA: Associates with OSMR to form the interleukin-31 receptor which activates STAT3 and to a lower extent STAT1 and STAT5. May function in skin immunity. Defects in IL31RA are the cause of amyloidosis primary localized cutaneous type 2 (PLCA2). PLCA2 is primary amyloidosis characterized by localized cutaneous amyloid deposition. This condition usually presents with itching (especially on the lower legs) and visible changes of skin hyperpigmentation and thickening that may be exacerbated by chronic scratching and rubbing. Primary localized cutaneous amyloidosis is often divided into macular and lichen subtypes although many affected individuals often show both variants coexisting. Lichen amyloidosis characteristically presents as a pruritic eruption of grouped hyperkeratotic papules with a predilection for the shins, calves, ankles and dorsa of feet and thighs. Papules may coalesce to form hyperkeratotic plaques that can resemble lichen planus, lichen simplex or nodular prurigo. Macular amyloidosis is characterized by small pigmented macules that may merge to produce macular hyperpigmentation, sometimes with a reticulate or rippled pattern. In macular and lichen amyloidosis, amyloid is deposited in the papillary dermis in association with grouped colloid bodies, thought to represent degenerate basal keratinocytes. The amyloid deposits probably reflect a combination of degenerate keratin filaments, serum amyloid P component, and deposition of immunoglobulins. Belongs to the type I cytokine receptor family. Type 2 subfamily. 12 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 5q11.2
Cellular Component: axon; cell junction; external side of plasma membrane; integral component of membrane; intracellular; plasma membrane; presynaptic membrane
Molecular Function: cytokine binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein kinase binding; transcription coactivator activity
Biological Process: acute inflammatory response to antigenic stimulus; cytokine-mediated signaling pathway; defense response; glandular epithelial cell differentiation; homeostatic process; JAK-STAT cascade; macrophage differentiation; MAPK cascade; monocyte differentiation; negative regulation of apoptosis; negative regulation of macrophage activation; positive regulation of cell proliferation; positive regulation of transcription, DNA-templated; positive regulation of tyrosine phosphorylation of STAT protein; transmembrane receptor protein tyrosine kinase signaling pathway
Disease: Amyloidosis, Primary Localized Cutaneous, 2
Research Articles on IL31RA
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Product Notes
The Human IL31RA il31ra (Catalog #AAA285709) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA285709 ELISA Kit recognizes Human IL31RA. It is sometimes possible for the material contained within the vial of "Interleukin-31, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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