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Human Interleukin-28 ELISA Kit | IL28RA elisa kit

Human Interleukin-28 receptor subunit alpha (IL28RA) ELISA Kit

Gene Names
IFNLR1; IFNLR; LICR2; IL28RA; CRF2/12; IL-28R1
Reactivity
Human
Synonyms
Interleukin-28; Human Interleukin-28 receptor subunit alpha (IL28RA) ELISA Kit; CRF2/12; IFNLR; IFNLR1; IL-28R1; LICR2; class II cytokine receptor CRF2/12|interferon lambda; receptor 1|interleukin 28 receptor A|interleukin 28 receptor; alpha|interleukin or cytokine receptor 2; IL28RA elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human IL28RA. No significant cross-reactivity or interference between Human IL28RA and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for IL28RA elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IL28RA in samples. An antibody specific for IL28RA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL28RA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL28RA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL28RA bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,536 Da
NCBI Official Full Name
interferon lambda receptor 1 isoform 1
NCBI Official Synonym Full Names
interferon lambda receptor 1
NCBI Official Symbol
IFNLR1
NCBI Official Synonym Symbols
IFNLR; LICR2; IL28RA; CRF2/12; IL-28R1
NCBI Protein Information
interferon lambda receptor 1
UniProt Protein Name
Interferon lambda receptor 1
UniProt Gene Name
IFNLR1
UniProt Synonym Gene Names
IL28RA; LICR2; IFN-lambda receptor 1; IFN-lambda-R1; CRF2-12; IL-28 receptor subunit alpha; IL-28R-alpha; IL-28RA; LICR2

NCBI Description

The protein encoded by this gene belongs to the class II cytokine receptor family. This protein forms a receptor complex with interleukine 10 receptor, beta (IL10RB). The receptor complex has been shown to interact with three closely related cytokines, including interleukin 28A (IL28A), interleukin 28B (IL28B), and interleukin 29 (IL29). The expression of all three cytokines can be induced by viral infection. The cells overexpressing this protein have been found to have enhanced responses to IL28A and IL29, but decreased response to IL28B. Three alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL28RA: The IL28RA/IL10RB dimer is a receptor for IL28A, IL28B and IL29. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Belongs to the type II cytokine receptor family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: integral component of membrane; interleukin-28 receptor complex; plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding

Biological Process: cytokine-mediated signaling pathway; defense response to virus; mucosal immune response; negative regulation of cell proliferation; regulation of defense response to virus by host

Research Articles on IL28RA

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Product Notes

The Human IL28RA ifnlr1 (Catalog #AAA285435) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA285435 ELISA Kit recognizes Human IL28RA. It is sometimes possible for the material contained within the vial of "Interleukin-28, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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