IL27Ra / TCCR / WSX1 recombinant protein

Recombinant Human IL27Ra / TCCR / WSX1 Protein (Fc tag)

Cat. Num. AAA2546224

• Gene Names: IL27RA; CRL1; TCCR; WSX1; IL27R; IL-27RA; zcytor1
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: IL27Ra / TCCR / WSX1; Recombinant Human IL27Ra / TCCR / WSX1 Protein (Fc tag); IL27RA; CRL1; IL27R; TCCR; WSX1; zcytor1; IL27Ra / TCCR / WSX1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 8% trehalose, 8% mannitol
• Sequence Length: 636

• Application Notes: The recombinant human IL27Ralpha/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 722 amino acids and has a predicted molecular mass of 79 kDa. As a result of glycosylation, rh IL27Ralpha/Fc monomer migrates as an approximately 105-115 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gln 33
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for IL27Ra / TCCR / WSX1 recombinant protein

Background: The interleukin-27 receptor is a type I cytokine receptor for interleukin-27. It is a heterodimer composed of the interleukin 27 receptor, alpha subunit and glycoprotein 130. WSX-1/IL-27R, a class I cytokine receptor that is homologous to the beta2 chain of the IL-12R in both sequence and structure, is highly expressed by resting/naive CD4+ T cells and CD8+ T cells. WSX-1/IL-27R belongs to the IL-6/IL-12 family of cytokines and induced proliferation of naive CD4 (+) T cells and the generation of a Th1-type adaptive immune response. WSX-1/IL-27R functions as a receptor for IL27. IL-27 not only contributes to the development of an adaptive immune response through its action on CD4 (+) T cells, it also directly acts on cells of the innate immune system. WSX-1/IL-27R requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. It is involved in the regulation of Th1-type immune responses. IL-27R also appears to be involved in innate defense mechanisms. IL27RA is essential for transcriptional activation of STAT1 and for augmenting the induction of T-bet expression during initiation of Th1 cell differentiation.

Description: A DNA sequence encoding the extracellular domain (Met 1-Arg 514) of human IL27Ralpha (NP_004834.1) was fused with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4759328
• NCBI GeneID: 9466
• NCBI Accession #: NP_004834.1
• NCBI GenBank Nucleotide #: NM_004843.3
• UniProt Accession #: Q6UWB1; O60624; A0N0L1
• Molecular Weight: 69,474 Da
• NCBI Official Full Name: interleukin-27 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 27 receptor subunit alpha
• NCBI Official Symbol: IL27RA
• NCBI Official Synonym Symbols: CRL1; TCCR; WSX1; IL27R; IL-27RA; zcytor1
• NCBI Protein Information: interleukin-27 receptor subunit alpha
• UniProt Protein Name: Interleukin-27 receptor subunit alpha
• UniProt Gene Name: IL27RA
• UniProt Synonym Gene Names: CRL1; TCCR; WSX1; IL-27 receptor subunit alpha; IL-27R subunit alpha; IL-27R-alpha; IL-27RA; TCCR

NCBI Description

In mice, CD4+ helper T-cells differentiate into type 1 (Th1) cells, which are critical for cell-mediated immunity, predominantly under the influence of IL12. Also, IL4 influences their differentiation into type 2 (Th2) cells, which are critical for most antibody responses. Mice deficient in these cytokines, their receptors, or associated transcription factors have impaired, but are not absent of, Th1 or Th2 immune responses. This gene encodes a protein which is similar to the mouse T-cell cytokine receptor Tccr at the amino acid level, and is predicted to be a glycosylated transmembrane protein. [provided by RefSeq, Jul 2008]

Uniprot Description

IL27RA: Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1- type immune responses. Also appears to be involved in innate defense mechanisms. Belongs to the type I cytokine receptor family. Type 2 subfamily.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: transmembrane receptor activity

Biological Process: cell surface receptor linked signal transduction; immune response