Interleukin-2 / IL-2 Recombinant Protein | IL-2 recombinant protein

Recombinant Mouse Interleukin-2 / IL-2 Protein

Cat. Num. AAA2546614

• Gene Names: Il2; Il-2
• Purity: > 92 % as determined by SDS-PAGE
• Synonyms: Interleukin-2 / IL-2; Recombinant Mouse Interleukin-2 / IL-2 Protein; DN-144H19.3; Il-2; IL-2 recombinant protein

• Host: E Coli
• Purity/Purification: > 92 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 2mM DTT, pH 7.4
• Sequence Length: 169

• Application Notes: The recombinant mouse IL2 consists of 150 amino acids and has a calculated molecular mass of 17.4KDa. It migrates as an approximately 19 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for IL-2 recombinant protein

Background: Interleukin-2, also known as T-cell growth factor, TCGF, Aldesleukin and IL2, is a secreted protein which belongs to the IL-2 family. Interleukin-2 / IL-2 was the first interleukin molecule to be discovered. Interleukin-2 / IL-2 molecule was first purified to homogeneity by immunoaffinity chromatography by Kendall Smith and his team at Dartmouth Medical School. Interleukin-2 / IL-2 was also the first cytokine shown to mediate its effects via a specific IL-2 receptor, and it was also the first interleukin to be cloned and expressed from a complementary DNA (cDNA) library. Interleukin-2 / IL-2 was designated number 2 because Smith's data at the time indicated that IL-1, produced by macrophages, facilitates IL-2 production by T lymphocytes (T cells). Interleukin-2 / IL-2 is produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Interleukin-2 / IL-2 is normally produced by the body during an immune response. When environmental substances (molecules or microbes) gain access to the body, these substances (termed antigens) are recognized as foreign by antigen receptors that are expressed on the surface of lymphocytes. Antigen binding to the T cell receptor (TCR) stimulates the secretion of Interleukin-2 / IL-2, and the expression of IL-2 receptors IL-2R. The IL-2 / IL-2R interaction then stimulates the growth, differentiation and survival of antigen-selected cytotoxic T cells via the activation of the expression of specific genes. Interleukin-2 / IL-2 can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. The World Reference Standard for Interleukin-2 / IL-2 is produced by the National Institute of Biological Standards and Control in the UK. A recombinant form of Interleukin-2 / IL-2 for clinical use is manufactured by Chiron Corporation with the brand name Proleukin. It has been approved by the Food and Drug Administration (FDA) for the treatment of cancers (malignant melanoma, renal cell cancer), and is in clinical trials for the treatment of chronic viral infections, and as a booster (adjuvant) for vaccines. The use of Interleukin-2 / IL-2 in HIV therapy has been found to be ineffective.

Description: A DNA sequence encoding the soluble form of mouse IL2 (P04351) (Ala 21-Gln 169) was expressed, with an initial Met at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 7110653
• NCBI GeneID: 16183
• NCBI Accession #: NP_032392.1
• NCBI GenBank Nucleotide #: NM_008366.3
• UniProt Accession #: P04351; P97945; Q791T3
• Molecular Weight: 19,400 Da
• NCBI Official Full Name: interleukin-2
• NCBI Official Synonym Full Names: interleukin 2
• NCBI Official Symbol: Il2
• NCBI Official Synonym Symbols: Il-2
• NCBI Protein Information: interleukin-2
• UniProt Protein Name: Interleukin-2
• UniProt Gene Name: Il2
• UniProt Synonym Gene Names: Il-2; IL-2; TCGF

Uniprot Description

IL2: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. Belongs to the IL-2 family.

Protein type: Cytokine; Oncoprotein; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3 B|3 18.3 cM

Cellular Component: extracellular space

Molecular Function: carbohydrate binding; cytokine activity; glycosphingolipid binding; interleukin-2 receptor binding; kappa-type opioid receptor binding

Biological Process: elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; negative regulation of B cell apoptosis; negative regulation of heart contraction; negative regulation of inflammatory response; negative regulation of lymphocyte proliferation; negative regulation of protein amino acid phosphorylation; positive regulation of activated T cell proliferation; positive regulation of B cell proliferation; positive regulation of immunoglobulin secretion; positive regulation of interferon-gamma production; positive regulation of interleukin-17 production; positive regulation of isotype switching to IgG isotypes; positive regulation of protein amino acid phosphorylation; positive regulation of regulatory T cell differentiation; positive regulation of T cell differentiation; positive regulation of T cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat5 protein; protein kinase C activation; regulation of T cell homeostatic proliferation; response to ethanol