Mouse Interleukin 1 Family, Member 9 ELISA Kit | IL1F9 elisa kit

Mouse Interleukin 1 Family, Member 9 ELISA Kit

Cat. Num. AAA83501

• Gene Names: IL36G; IL1E; IL1F9; IL1H1; IL-1F9; IL-1H1; IL1RP2; IL-1RP2
• Reactivity: Mouse
• Synonyms: Interleukin 1 Family; Member 9; Mouse Interleukin 1 Family; Member 9 ELISA Kit; IL1F9 elisa kit

• Reactivity: Mouse
• Specificity: No significant cross-reactivity or interference between Mouse IL1F9 and analogues was observed.
• Sequence Length: 169

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 6.25 pg/ml - 200 pg/ml.
• Sensitivity: 1.0 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated IL1F9 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse IL1F9 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL1F9 and IL1F9 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL1F9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for IL1F9 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated IL1F9 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse IL1F9 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL1F9 and IL1F9 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL1F9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 9665234
• NCBI GeneID: 56300
• NCBI Accession #: NP_062564.1
• NCBI GenBank Nucleotide #: NM_019618.3
• UniProt Accession #: Q9NZH8; Q56B91; Q6UVX7; Q7RTZ9
• Molecular Weight: 18,721 Da
• NCBI Official Full Name: interleukin-36 gamma isoform 1
• NCBI Official Synonym Full Names: interleukin 36, gamma
• NCBI Official Symbol: IL36G
• NCBI Official Synonym Symbols: IL1E; IL1F9; IL1H1; IL-1F9; IL-1H1; IL1RP2; IL-1RP2
• NCBI Protein Information: interleukin-36 gamma; IL-1 epsilon; IL-1-epsilon; IL-1(EPSILON); interleukin-1 epsilon; IL-1 related protein 2; IL-1-related protein 2; interleukin-1 homolog 1; interleukin-1 family member 9; interleukin 1 family, member 9; interleukin 1-related protein 2
• UniProt Protein Name: Interleukin-36 gamma
• UniProt Gene Name: IL36G
• UniProt Synonym Gene Names: IL1E; IL1F9; IL1H1; IL1RP2; IL-1RP2; IL-1 epsilon; IL-1F9; IL-1H1
• UniProt Entry Name: IL36G_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2 (IL1RL2/IL1R-rp2), and is specifically inhibited by interleukin 1 family, member 5 (IL1F5/IL-1 delta). Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

Il1f9: Function as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1), that is present in epithelial barriers and takes part in local inflammatory response. Belongs to the IL-1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 2q12-q21

Cellular Component: extracellular space

Molecular Function: interleukin-1 receptor binding; cytokine activity

Biological Process: cell-cell signaling; cytokine and chemokine mediated signaling pathway; innate immune response; positive regulation of interleukin-6 production; negative regulation of myoblast differentiation; inflammatory response