Human Vascular Endothelial Growth Inhibitor (VEGI) ELISA Kit | VEGI elisa kit

Human Vascular Endothelial Growth Inhibitor (VEGI) ELISA Kit

Cat. Num. AAA2704560

• Gene Names: TNFSF15; TL1; TL1A; VEGI; TNLG1B; VEGI192A
• Reactivity: Human
• Synonyms: Vascular Endothelial Growth Inhibitor (VEGI); Human Vascular Endothelial Growth Inhibitor (VEGI) ELISA Kit; TNFSF15; TL1; TL1A; VEGI192A; Tumor Necrosis Factor(ligand)Superfamily; Member 15; TNF Superfamily Ligand TL1A; TNF Ligand-Related Molecule 1; VEGI elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Inhibitor (VEGI).; No significant cross-reactivity or interference between Vascular Endothelial Growth Inhibitor (VEGI) and analogues was observed.
• Sequence Length: 174

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 62.5-4000pg/mL
• Sensitivity: < 26.8pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Endothelial Growth Inhibitor (VEGI) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Endothelial Growth Inhibitor (VEGI) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VEGI elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Inhibitor (VEGI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Inhibitor (VEGI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Endothelial Growth Inhibitor (VEGI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vascular Endothelial Growth Inhibitor (VEGI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4038081
• NCBI GeneID: 9966
• Molecular Weight: 20,131 Da
• NCBI Official Full Name: vascular endothelial cell growth inhibitor
• NCBI Official Synonym Full Names: TNF superfamily member 15
• NCBI Official Symbol: TNFSF15
• NCBI Official Synonym Symbols: TL1; TL1A; VEGI; TNLG1B; VEGI192A
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 15
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 15
• UniProt Gene Name: TNFSF15
• UniProt Synonym Gene Names: TL1; VEGI

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

TNFSF15: Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis. Belongs to the tumor necrosis factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q32

Cellular Component: extracellular space; integral component of membrane; integral component of plasma membrane; intracellular; plasma membrane

Molecular Function: cytokine activity; receptor binding; tumor necrosis factor receptor binding

Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; activation of NF-kappaB-inducing kinase; apoptosis; cytokine metabolic process; immune response; signal transduction; tumor necrosis factor-mediated signaling pathway