Typical Testing Data/Standard Curve
Rat IL-1 alpha ELISA Kit | IL1A elisa kit
Rat IL-1 alpha ELISA Kit
Gene Names
Il1a; IL-1 alpha
Reactivity
Rat
Applications
ELISA
Synonyms
IL-1 alpha; Rat IL-1 alpha ELISA Kit; IL1F1; Interleukin-1 alpha; Hematopoietin-1; IL1A elisa kit
Reactivity
Rat
Specificity
The Rat IL-1 alpha ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat IL-1 alpha proteins within the range of 15.6 pg/ml - 1000 pg/ml.
Sequence Length
270
Applicable Applications for IL1A elisa kit
Sandwich ELISA (EIA)
Samples
Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates.
Sensitivity
7 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for IL1A elisa kit
Principle of the Assay||The Rat IL-1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-1 alpha in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Rat IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin (IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis.
Background/Introduction: Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin (IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,856 Da
NCBI Official Full Name
Interleukin-1 alpha
NCBI Official Synonym Full Names
interleukin 1 alpha
NCBI Official Symbol
Il1a
NCBI Official Synonym Symbols
IL-1 alpha
NCBI Protein Information
interleukin-1 alpha
UniProt Protein Name
Interleukin-1 alpha
Protein Family
UniProt Gene Name
Il1a
UniProt Synonym Gene Names
IL-1 alpha
UniProt Entry Name
IL1A_RAT
NCBI Description
proinflammatory cytokine [RGD, Feb 2006]
Uniprot Description
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Research Articles on IL1A
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Product Notes
The Rat IL1A il1a (Catalog #AAA8244575) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8244575 ELISA Kit recognizes Rat IL1A. AAA Biotech's IL-1 alpha can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (EIA). Researchers should empirically determine the suitability of the IL1A il1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-1 alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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