SDS-Page
Interleukin 18 Binding Protein (IL18BP) Recombinant Protein | IL18BP recombinant protein
Recombinant Interleukin 18 Binding Protein (IL18BP)
Gene Names
Il18bp; MC54L; Igifbp; IL-18BP
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Interleukin 18 Binding Protein (IL18BP); Recombinant Interleukin 18 Binding Protein (IL18BP); IL18BP recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-TS APQTTATVLT GSSKDPCSSW SPAVPTKQYP ALDVIWPEKE VPLNGTLTLS CTACSRFPYF SILYWLGNGS FIEHLPGRLK EGHTSREHRN TSTWLHRALV LEELSPTLRS TNFSCLFVDP GQVAQYHIIL AQLWDGLKTA PSPSQETLSS HSPVSRSAGP GVA
MGHHHHHHSGSEF-TS APQTTATVLT GSSKDPCSSW SPAVPTKQYP ALDVIWPEKE VPLNGTLTLS CTACSRFPYF SILYWLGNGS FIEHLPGRLK EGHTSREHRN TSTWLHRALV LEELSPTLRS TNFSCLFVDP GQVAQYHIIL AQLWDGLKTA PSPSQETLSS HSPVSRSAGP GVA
Sequence Length
193
Applicable Applications for IL18BP recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Thr29~Ala193 (Accession # Q9Z0M9) with N-terminal His-Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19.5kDa
NCBI Official Full Name
interleukin-18-binding protein
NCBI Official Synonym Full Names
interleukin 18 binding protein
NCBI Official Symbol
Il18bp
NCBI Official Synonym Symbols
MC54L; Igifbp; IL-18BP
NCBI Protein Information
interleukin-18-binding protein; interferon gamma inducing factor binding protein; interferon gamma-inducing factor-binding protein
UniProt Protein Name
Interleukin-18-binding protein
Protein Family
UniProt Gene Name
Il18bp
UniProt Synonym Gene Names
Igifbp; IL-18BP
UniProt Entry Name
I18BP_MOUSE
Uniprot Description
Function: Binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response
By similarity.
Subcellular location: Secreted
Potential.
Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Sequence caution: The sequence AAD17193.1 differs from that shown. Reason: Intron retention.The sequence AAD17194.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on IL18BP
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Product Notes
The IL18BP il18bp (Catalog #AAA2011434) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Interleukin 18 Binding Protein (IL18BP) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the IL18BP il18bp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF-TS APQTTATVLT GSSKDPCSSW SPAVPTKQYP ALDVIWPEKE VPLNGTLTLS CTACSRFPYF SILYWLGNGS FIEHLPGRLK EGHTSREHRN TSTWLHRALV LEELSPTLRS TNFSCLFVDP GQVAQYHIIL AQLWDGLKTA PSPSQETLSS HSPVSRSAGP GVA. It is sometimes possible for the material contained within the vial of "Interleukin 18 Binding Protein (IL18BP), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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