Interleukin 16 (IL16) Recombinant Protein | IL16 recombinant protein

Recombinant Interleukin 16 (IL16)

Cat. Num. AAA2018333

• Gene Names: IL16; LCF; NIL16; PRIL16; prIL-16
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Interleukin 16 (IL16); Recombinant Interleukin 16 (IL16); IL16 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MPDLNSST DSAASASAAS DVSVESTAEA TVCTVTLEKM SAGLGFSLEG GKGSLHGDKP LTINRIFKGA ASEQSETVQP GDEILQLGGT AMQGLTRFEA WNIIKALPDG PVTIVIRRKS LQSKETTAAG DS
• Sequence Length: 1331

• Applicable Applications for IL16 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Met1203~Ser1332 (Accession # Q14005) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 289063395
• NCBI GeneID: 3603
• NCBI Accession #: NP_001165599.1
• NCBI GenBank Nucleotide #: NM_001172128.1
• UniProt Accession #: Q14005; Q16435; Q6VVE6; Q6ZMQ7; Q9UP18; A6NM20; A8MU65; B9EGR6
• Molecular Weight: 17.2kDa
• NCBI Official Full Name: pro-interleukin-16 isoform 3
• NCBI Official Synonym Full Names: interleukin 16
• NCBI Official Symbol: IL16
• NCBI Official Synonym Symbols: LCF; NIL16; PRIL16; prIL-16
• NCBI Protein Information: pro-interleukin-16; prointerleukin 16; neuronal interleukin 16; lymphocyte chemoattractant factor
• UniProt Protein Name: Pro-interleukin-16
• Protein Family: Pro-interleukin
• UniProt Gene Name: IL16
• UniProt Synonym Gene Names: IL-16; LCF
• UniProt Entry Name: IL16_HUMAN

NCBI Description

The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

Uniprot Description

IL16: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: DNA-binding; Cytokine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: extracellular space; cytoplasm; plasma membrane; nucleus

Molecular Function: cytokine activity

Biological Process: viral reproduction; transcription, DNA-dependent; regulation of transcription, DNA-dependent; leukocyte chemotaxis; immune response; induction of positive chemotaxis