Sequence
Dihydroorotate Dehydrogenase (DHODH) Active Protein | DHODH active protein
Active Dihydroorotate Dehydrogenase (DHODH)
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence
Sequence
Activity
(Dihydroorotate dehydrogenase (DHODH) is an enzyme which catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. As an enzyme associated with the electron transport chain, DHODH could link mitochondrial bioenergetics, cell proliferation, ROS production, and apoptosis in certain cell types. DHODH depletion also resulted in increased ROS production, decreased membrane potential and cell growth retardation. Besides, FK506 Binding Protein 8 (FKBP8) has been identified as an interactor of DHODH, thus a binding ELISA assay was conducted to detect the interaction of recombinant human DHODH and recombinant human FKBP8. Briefly, DHODH were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FKBP8-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti- DHODH pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of DHODH and FKBP8 was shown in Figure 1, and this effect was in a dose dependent manner.Figure 1. The binding activity of DHODH with FKBP8)
Activity
(Dihydroorotate dehydrogenase (DHODH) is an enzyme which catalyzes the fourth
enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de
novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the
outer surface of the inner mitochondrial membrane. As an enzyme associated with
the electron transport chain, DHODH could link mitochondrial bioenergetics, cell
proliferation, ROS production, and apoptosis in certain cell types. DHODH depletion
also resulted in increased ROS production, decreased membrane potential and cell
growth retardation. Besides, FK506 Binding Protein 8 (FKBP8) has been identified
as an interactor of DHODH, thus a binding ELISA assay was conducted to detect the
interaction of recombinant human DHODH and recombinant human FKBP8. Briefly,
DHODH were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of
100uL were then transferred to FKBP8-coated microtiter wells and incubated for 2h
at 37 degree C. Wells were washed with PBST and incubated for 1h with anti- DHODH pAb,
then aspirated and washed 3 times. After incubation with HRP labelled secondary
antibody, wells were aspirated and washed 3 times. With the addition of substrate
solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution
to the wells and read at 450nm immediately. The binding activity of DHODH and
FKBP8 was shown in Figure 1, and this effect was in a dose dependent manner.Figure 1. The binding activity of DHODH with FKBP8)
SDS-PAGE
(Figure 2. SDS-PAGESample: Active recombinant DHODH, Human)
SDS-PAGE
(Figure 2. SDS-PAGESample: Active recombinant DHODH, Human)
Western Blot (WB)
(Figure 3. Western Blot Sample: Recombinant DHODH, Human; Antibody: Rabbit Anti-Human DHODH Ab (MBS2027983))
Western Blot (WB)
(Figure 3. Western Blot Sample: Recombinant DHODH, Human; Antibody: Rabbit Anti-Human DHODH Ab (MBS2027983))
NCBI and Uniprot Product Information
Accurate Molecular Mass: 42kDa as determined by SDS-PAGE reducing conditions.
NCBI Description
The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. [provided by RefSeq, Jul 2008]
Uniprot Description
DHODH: Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS); also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases. Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.
Protein type: Mitochondrial; EC 1.3.5.2; Nucleotide Metabolism - pyrimidine; Oxidoreductase; Membrane protein, integral
Chromosomal Location of Human Ortholog: 16q22
Cellular Component: nucleoplasm; mitochondrion; cell soma; mitochondrial inner membrane; integral to membrane; intrinsic to mitochondrial inner membrane
Molecular Function: ubiquinone binding; FMN binding; dihydroorotate dehydrogenase activity; drug binding
Biological Process: lactation; response to starvation; response to drug; 'de novo' pyrimidine base biosynthetic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; positive regulation of apoptosis; pyrimidine nucleoside biosynthetic process; female pregnancy; response to caffeine
Disease: Postaxial Acrofacial Dysostosis
Research Articles on DHODH
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Product Notes
The DHODH dhodh (Catalog #AAA2105191) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Active Dihydroorotate Dehydrogenase (DHODH) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Dihydroorotate Dehydrogenase (DHODH) can be used in a range of immunoassay formats including, but not limited to, Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the DHODH dhodh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dihydroorotate Dehydrogenase (DHODH), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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