Mouse IL-13 ELISA Kit | IL13 elisa kit

Mouse IL-13 PicoKine ELISA Kit

Cat. Num. AAA175905

• Gene Names: Il13; Il-13
• Reactivity: Mouse
• Synonyms: IL-13; Mouse IL-13 PicoKine ELISA Kit; Interleukin-13; interleukin 13; ALRH ; BHR 1; BHR1; Bronchial hyperresponsiveness 1 (bronchial asthma); IL 13; Il13; IL13_HUMAN; Interleukin 13; Interleukin13 ; MGC116786; MGC116788; MGC116789; NC30; P600; IL13 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Il13
• Sequence Length: 131

• Samples: Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
• Detection Range: 15.6 pg/ml - 1,000 pg/ml
• Sensitivity: <2 pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for IL13 elisa kit

Principle of the Assay: The Picokine™ Mouse Il13 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Il13 with a 96-well strip plate that is pre-coated with antibody specific for Il13. The detection antibody is a biotinylated antibody specific for Il13. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Il13 with immunogen: Expression system for standard: E.coli; Immunogen sequence: S26-F131. The kit is analytically validated with ready-to-use reagents. To measure Mouse Il13, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Il13 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Il13 in the sample.

Background/Introduction: Interleukin (IL)-13 is a major inducer of fibrosis in many chronic infectious and autoimmune diseases. Recombinant IL-13 protein inhibits inflammatory cytokine production induced by lipopolysaccharide in human peripheral blood monocytes. Moreover, it synergizes with IL-13 in regulating interferon-gamma synthesis in large granular lymphocytes. Interleukin-13 may be critical in regulating inflammatory and immune responses. IL-13 and IL-4 are two cytokines produced by T helper type 2 cells, mast cells, and basophils. In addition to their physiological roles, these cytokines are also implicated in pathological conditions such as asthma and allergy. The genes encoding human IL-4 and IL-13 are located on segment q23-31 of chromosome 5.4 Recombinant mouse IL-13 is a mixture of Ser26-Phe131 amino acids with the molecular mass of 11.5KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 129377
• NCBI GeneID: 16163
• UniProt Accession #: P20109
• Molecular Weight: 14,108 Da
• NCBI Official Full Name: Interleukin-13
• NCBI Official Synonym Full Names: interleukin 13
• NCBI Official Symbol: Il13
• NCBI Official Synonym Symbols: Il-13
• NCBI Protein Information: interleukin-13; T-cell activation protein P600
• UniProt Protein Name: Interleukin-13
• Protein Family: Interleukin
• UniProt Gene Name: Il13
• UniProt Synonym Gene Names: Il-13; IL-13
• UniProt Entry Name: IL13_MOUSE

Uniprot Description

IL13: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Defects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH). Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure. Belongs to the IL-4/IL-13 family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular space; cytoplasm; extracellular region; external side of plasma membrane

Molecular Function: interleukin-13 receptor binding; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity

Biological Process: response to nicotine; positive regulation of smooth muscle cell proliferation; microglial cell activation; regulation of proton transport; positive regulation of mast cell degranulation; positive regulation of connective tissue growth factor production; positive regulation of tyrosine phosphorylation of Stat6 protein; positive regulation of immunoglobulin production; positive regulation of protein secretion; positive regulation of B cell proliferation; immune response; positive regulation of ion transport; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of NAD(P)H oxidase activity; inflammatory response; positive regulation of macrophage activation